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August 26, 2020

Formation of N-nitrosodimethylamine During the storage of Ranitidine reagent powders and tablets

By Danielle

Temperature-dependent formation of N-nitrosodimethylamine during the storage of ranitidine reagent powders and tablets

The purpose of this study was to elucidate the effect of high-temperature storage on the stability of ranitidine, specifically with respect to the potential formation of N-nitrosodimethylamine (NDMA), which is classified as a probable human carcinogen.
Commercially available ranitidine reagent powders and formulations were stored under various conditions, and subjected to LC-MS/MS analysis. When ranitidine tablets from two different brands (designated as tablet A and tablet B) were stored under accelerated condition (40°C with 75% relative humidity), following the drug stability guidelines issued by the International Conference on Harmonisation (ICH-Q1A), for up to 8 weeks, the amount of NDMA in them substantially increased from 0.19 to 116 ppm and from 2.89 to 18 ppm, respectively.
  • The formation of NDMA that exceeded the acceptable daily intake limit (0.32 ppm) at the temperature used under accelerated storage conditions clearly highlights the risk of NDMA formation in ranitidine formulations when extrapolated to storage under ambient conditions.
  • A forced-degradation study under the stress condition (60°C for 1 week) strongly suggested that environmental factors such as moisture and oxygen are involved in the formation of NDMA in ranitidine formulations.
  • Storage of ranitidine tablets and reagent powders at the high temperatures also increased the amount of nitrite, which is considered one of the factors influencing NDMA formation. These data indicate the necessity of controlling/monitoring stability-related factors, in addition to controlling impurities during the manufacturing process, in order to mitigate nitrosamine-related health risks of certain pharmaceuticals.

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TaqProbe 2X qPCR MasterMix-ROX
MasterMix-P 4 x 1.25 ml for 500 reactions (20 ul)
EUR 140

TaqProbe 5X qPCR MasterMix-No Dye
MasterMix-5PS 4 x 1.0 ml for 1000 reactions (20 ul)
EUR 213

BrightGreen 5X qPCR MasterMix-ROX
MasterMix-5R 4 x 1.0 ml for 1000 reactions (20 ul)
EUR 213

KiloGreen 2X qPCR MasterMix-Low ROX
MasterMix-KL 4 x 1.25 ml - 500 reactions (20 ul)
EUR 140

BrightGreen 2X qPCR MasterMix-Low ROX
MasterMix-LR 4 x 1.25 ml for 500 reactions (20 ul)
EUR 140

BrightGreen miRNA qPCR MasterMix-Low ROX
MasterMix-mL 4 x 1.25 ml for 500 reactions
EUR 151

TaqProbe 2X qPCR MasterMix-iCycler
MasterMix-PC 4 x 1.25 ml for 500 reactions (20 ul)
EUR 140

TaqProbe 2X qPCR MasterMix-Multiplex
MasterMix-PM 4 x 1.25 ml for 500 reactions (20 ul)
EUR 140

BrightGreen 2X qPCR MasterMix-Low ROX
MasterMix-LR-XL 16 x 1.25 ml for 2000 reactions (20 ul)
EUR 376

BrightGreen Express 2X qPCR MasterMix-Low ROX
MasterMix-EL 4 x 1.25 ml for 500 reactions (20 ul)
EUR 140

BrightGreen 5X qPCR MasterMix-iCycler
MasterMix-5C 4 x 1.0 ml for 1000 reactions (20 ul)
EUR 213

TaqProbe 2X qPCR MasterMix-No Dye
MasterMix-PS 4 x 1.25 ml for 500 reactions (20 ul)
EUR 140

KiloGreen 2X qPCR MasterMix-ROX
MasterMix-KR 4 x 1.25 ml - 500 reactions (20 ul)
EUR 140

BrightGreen miRNA qPCR MasterMix-ROX
MasterMix-mR 4 x 1.25 ml for 500 reactions
EUR 151

BrightGreen 2X qPCR MasterMix-ROX
MasterMix-R 4 x 1.25 ml for 500 reactions (20 ul)
EUR 140

BrightGreen 5X qPCR MasterMix-No Dye
MasterMix-5S 4 x 1.0 ml for 1000 reactions (20 ul)
EUR 213

BrightGreen 2X qPCR MasterMix-ROX
MasterMix-R-XL 16 x 1.25 ml for 2000 reactions (20 ul)
EUR 376

BrightGreen Express 2X qPCR MasterMix-ROX
MasterMix-ER 4 x 1.25 ml for 500 reactions (20 ul)
EUR 140

BrightGreen 2X qPCR MasterMix-iCycler
MasterMix-iC 4 x 1.25 ml for 500 reactions (20 ul)
EUR 140

KiloGreen 2X qPCR MasterMix-iCycler
MasterMix-KC 4 x 1.25 ml - 500 reactions (20 ul)
EUR 140

BrightGreen miRNA qPCR MasterMix-iCycler
MasterMix-mC 4 x 1.25 ml for 500 reactions
EUR 151

Green-2-Go qPCR Mastermix-Low ROX (500X20ul Rxn)
QPCR002-L 4X1.25ml
EUR 252.71

BrightGreen Express 2X qPCR MasterMix-iCycler
MasterMix-EC 4 x 1.25 ml for 500 reactions (20 ul)
EUR 140

KiloGreen 2X qPCR MasterMix-No Dye
MasterMix-KS 4 x 1.25 ml - 500 reactions (20 ul)
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BrightGreen miRNA qPCR MasterMix-No Dye
MasterMix-mS 4 x 1.25 ml for 500 reactions
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BrightGreen 2X qPCR MasterMix-No Dye
MasterMix-S 4 x 1.25 ml for 500 reactions (20 ul)
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BrightGreen 2X qPCR MasterMix-iCycler
MasterMix-iC-XL 16 x 1.25 ml for 2000 reactions (20 ul)
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BrightGreen Express 2X qPCR MasterMix-No Dye
MasterMix-ES 4 x 1.25 ml for 500 reactions (20 ul)
EUR 140

BrightGreen 2X qPCR MasterMix-No Dye
MasterMix-S-XL 16 x 1.25 ml for 2000 reactions (20 ul)
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Bio-Star Mastermix (2X) Low-ROX
P150 100 rcs (2,5 ml)
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One-Step TaqProbe qRT-PCR-Low ROX
G493-PL 100 rxn (20 ul/rxn)
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Green-2-Go qPCR Mastermix-ROX (500X20ul Rxn)
QPCR001-R 4X1.25ml
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Green-2-Go TaqProbe qPCR Mastermix-no Dye (500X20ul Rxn)
QPCR005-NODYE 4X1.25ml
EUR 252.71

ExCellenCT One-Step TaqProbe qRT-PCR-Low ROX
G918-PL 25 Preps 100 x 20 ul reactions
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SYBR Green qPCR Master Mix (Low ROX)
HY-K0522 5 mL (500 rxns )
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1730 5X SNAP-SEAL 13ML
1730-5X 500/pk
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Description: Disposable Plastic; Plastic Containers

Genious 2X SYBRGREEN FAST qPCR MIX (Low ROX)
RK21206 1 ml Ask for price

2x SYBR Green qPCR Master Mix (Low ROX)
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Description: 2x SYBR Green qPCR master mix (Low ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR

2x SYBR Green qPCR Master Mix (Low ROX)
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Description: 2x SYBR Green qPCR master mix (Low ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR

One-Step TaqProbe qRT-PCR-ROX
G493-P 100 rxn (20 ul/rxn)
EUR 158

Green-2-Go 1-Step qRT-PCR Mastermix-Low Rox
QPCR012-LR 100Rxn
EUR 239.23

5X All-In-One RT MasterMix
G485 25 x 10 ul reactions
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5X All-In-One RT MasterMix
G486 100 x 10 ul reactions
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5X All-In-One RT MasterMix
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5X All-In-One RT MasterMix
G590 1000 x 10 ul reactions
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ExCellenCT One-Step TaqProbe qRT-PCR-ROX
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HotTaq Probe qPCR Mix (ROX)
BT11001 250rxn
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Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq EvaGreen qPCR Mix (ROX)
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Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

Fast Plus EvaGreen qPCR Master Mix with low Rox (500 rxn)
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Description: Minimum order quantity: 1 unit of 5x1mL

Fast Plus EvaGreen qPCR Master Mix with low Rox (5000 rxn)
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Forget-Me-Notâ„¢ EvaGreen qPCR Master Mix (Low ROX) (500 reactions)
9-31045
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Description: Minimum order quantity: 1 unit of 5x1mL

HotTaq Probe qPCR Mix (no ROX)
BT11002 250rxn
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Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq EvaGreen qPCR Mix (no ROX)
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Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

Pro QPCR SuperMix Kit - ROX premixed
K5056200 200 reactions
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Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Pro QPCR SuperMix Kit - ROX premixed
K5056400 400 reactions
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Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Green-2-Go qPCR Mastermix (500X20ul Rxn)
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5X All-In-One RT MasterMix (25x10ul Rxns)
HRT025-10 25x10ulRxns
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5X All-In-One RT MasterMix (100x10ul Rxns)
HRT100-10 100x10ulRxns
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5X All-In-One RT MasterMix (200x10ul Rxns)
HRT200-10 200x10ulRxns
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Jade? Mix-Low ROX
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Fast Plus EvaGreen qPCR Master Mix with low Rox (100 rxn): (1mL)
31014-T 1mL
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Description: Minimum order quantity: 1 unit of 1mL

Real-Time PCR Mastermix 2x, Low Level of Rox , 100RXNs, optimized ready-to-use mastermix containing unique reaction buffer, dNTP, Hot-Start Taq DNA polymerase, and QPCR dye
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Green-2-Go qPCR Mastermix-iCycler (500X20ul Rxn)
QPCR003-iC 4X1.25ml
EUR 252.71

SYBR Green qPCR Master Mix (High ROX)
HY-K0521 1 mL (100 rxns)
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SYBR Green qPCR Master Mix (No ROX)
HY-K0523 5 mL (500 rxns )
EUR 257

ROX reference dye for qPCR, 1 mL
41110 1 mL
EUR 52

Accuris qMax Probe No Rox qPCR Mix
PR2001-N-1000 1 PC
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ROX reference dye for qPCR, 500 uL
31110 500 uL
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Fast Pro QPCR SuperMix Kit - ROX premixed
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Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Fast Pro QPCR SuperMix Kit - ROX premixed
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Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

SensiMix SYBR Low-ROX Kit
QT625-05 500 rxn Ask for price

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QT625-20 2000 rxn Ask for price

Jade Express? Mix-Low ROX
M1111-500
EUR 441

Jade Smart? Mix-Low ROX
M1115-500
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Jade miRNA? Mix-Low ROX
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Taqman Master Mix-Low ROX
M1123-500
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Jade? QRT Kit-Low ROX
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Taqman QRT Kit-Low ROX
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EUR 370

Bifunctional Reagents for Formylglycine Conjugation: Pitfalls and Breakthroughs

Formylglycine-generating enzymes specifically oxidize cysteine within the consensus sequence CxPxR to C α -formylglycine (FGly). This non-canonical electrophilic amino acid can subsequently be addressed selectively via bioorthogonal Hydrazino- iso -Pictet-Spengler (HIPS) or Knoevenagel ligation to attach payloads like fluorophores or drugs to proteins, while obtaining a defined payload-to-protein ratio. However, disadvantages of these conjugation techniques include the need of a large excess of conjugation building block, comparably low reaction rates and limited stability of FGly-containing proteins.
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  • Furthermore, an azide-modified tandem Knoevenagel building block enabled the synthesis of branched PEG-linkers and the conjugation of two fluorophores, resulting in an improved signal-to-noise ratio in live cell fluorescence imaging experiments targeting the EGF-receptor.

Buzz about RT-qPCR: An RT-qPCR formulation for SARS-CoV-2 detection using reagents produced at Georgia Institute of Technology

Widespread testing for the presence novel coronavirus SARS-CoV-2 in patients remains vital for controlling the COVID-19 pandemic prior to the advent of an effective treatment.
  • The early testing shortfall in some parts of the US can be traced to an initial shortage of supplies, expertise and/or instrumentation necessary to detect the virus by quantitative reverse transcription polymerase chain reaction (RT-qPCR). Here we show that academic biochemistry and molecular biology laboratories equipped with appropriate expertise and infrastructure can produce the RT-qPCR assay and backfill pipeline shortages.
  • The Georgia Tech COVID-19 Test Kit Support Group synthesized multiplexed primers and probes and formulated a master mix composed of enzymes and proteins produced in-house. We compare the performance of our in-house kit to a commercial product used for diagnostic testing and describe implementation of environmental testing to monitor surfaces across various campus laboratories for the presence of SARS-CoV-2.

Design and scalable synthesis of novel N-alkyl-hydroxylamine reagents for the direct, Fe-catalyzed installation of medicinally relevant amines

Secondary and tertiary alkylamines are privileged substance classes which are often found in pharmaceuticals and other biologically active small molecules. Herein, we report their direct synthesis from alkenes through an aminative difunctionalization reaction enabled by iron catalysis.

A family of ten novel hydroxylamine-derived aminating reagents were designed for the installation of several medicinally relevant amine groups, such as methylamine, morpholine and piperazine, through the aminochlorination of alkenes.

The methodology displays an excellent functional group tolerance, and a broad scope of alkenes was converted to the corresponding products, including several drug-like molecules. Besides aminochlorination, the installation of other functionalities through aminoazidation, aminohydroxylation and even intramolecular carboamination reactions, is demonstrated, further highlighting the broad potential of these new reagents for the discovery of novel amination reactions.

PinPoint-HR System for Platform Cell Line Generation & Retargeting of AAVS1 Safe Harbor Locus (includes PIN410A-1, GE601A-1, PIN200A-1, PIN510A-1, & PIN600A-1)
PIN410A-KIT 1 Kit
EUR 4335

PinPoint-HR System for Platform Cell Line Generation & Retargeting of AAVS1 Safe Harbor Locus (includes PIN410A-1, CAS601A-1, PIN200A-1, PIN510A-1, & PIN600A-1)
PIN412A-KIT 1 Kit
EUR 4335

Up-Regulator of Cell Proliferation (URGCP) Antibody
abx146141-100ug 100 ug
EUR 391

Human Up-regulator of cell proliferation (URGCP) ELISA Kit
abx392266-96tests 96 tests
EUR 911

Mouse Up- regulator of cell proliferation, Urgcp ELISA KIT
ELI-22211m 96 Tests
EUR 865

Bovine Up- regulator of cell proliferation, URGCP ELISA KIT
ELI-17774b 96 Tests
EUR 928

Human Up- regulator of cell proliferation, URGCP ELISA KIT
ELI-17775h 96 Tests
EUR 824

Human p53 up- regulated modulator of apoptosis ELISA Kit
ELA-E1909h 96 Tests
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PCR Clean Up for DNA Sequencing
BT5100 100preps
EUR 95.68

PCR Clean Up for DNA Sequencing
BT5101 1000Preps, 1000prep
EUR 461.08

Rack for up to 3 microplates
BV1010-MP 1 PC
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Accuris Hot Start Taq
PR1000-HS-1000 1 PC
EUR 305.05

Accuris Hot Start Taq
PR1000-HS-500 1 PC
EUR 204.56

Accuris Hot Start Taq
PR1000-HS-6000 1 PC
EUR 1225.36

Accuris Hot Start Taq
PR1000-HS-S 1 PC
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Fire Start? PCR Mix
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Fire Start? DNA Polymerase
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EUR 637

Fire Start? DNA Polymerase
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Human p53 up-regulated modulator of apoptosis(PUMA)ELISA Kit
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Human p53 up-regulated modulator of apoptosis(PUMA)ELISA Kit
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EUR 466

Human p53 up-regulated modulator of apoptosis,PUMA ELISA Kit
201-12-1820 96 tests
EUR 440
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Human p53 up-regulated modulator of apoptosis(PUMA)ELISA Kit
QY-E04714 96T
EUR 361

Bradford(Coomassie) Protein Assay Plus Reagents
P7201-050 450ml
EUR 188

PinPoint-FC 293T Platform Kit for Targeted Gene Insertion (includes PIN320A-1, PIN200A-1, PIN510A-1 & PIN600A-1)
PIN320A-KIT 1 Kit
EUR 4941

CLOuD9 Gene Expression Regulation Kit (includes 10 ug each of dCas9-PYL1 and dCas9-ABI1 lentivectors, and 100 ul of 0.5M Inducer Agent)
CASCL9-100A-KIT 1 Kit
EUR 1132

PinPoint-FC Murine iPSC Platform Kit for Targeted Gene Insertion (includes PIN340iPS-1, PIN200A-1, PIN510A-1 & PIN600A-1)
PIN340iPS-KIT 1 Kit
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Universal platform with adjustable bars, 2 x 1000mL flasks or 4 x 500mL flasks or 4 x 250mL flasks for UniPact?
ACT-USI-UP
EUR 532

PCR Clean-up Kit,
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PCR Clean-up Kit,
PC100 100 preps
EUR 130

PCR Clean-up Kit,
PC50 50 preps
EUR 94

Rabbit UP ELISA Kit
ERTU0018 96Tests
EUR 521

Rat UP ELISA Kit
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Mouse UP ELISA Kit
EMU0018 96Tests
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Porcine UP ELISA Kit
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Goat UP ELISA Kit
EGTU0018 96Tests
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Human UP ELISA Kit
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Bovine UP ELISA Kit
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Canine UP ELISA Kit
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Anserine UP ELISA Kit
EAU0018 96Tests
EUR 521

Up RNA Extraction Kit
20-abx098087
  • EUR 258.00
  • EUR 105.00
  • 100 ml
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CLIA kit for Human HYOU1 (Hypoxia Up Regulated 1)
E-CL-H0346 1 plate of 96 wells
EUR 584
Description: A sandwich CLIA kit for quantitative measurement of Human HYOU1 (Hypoxia Up Regulated 1) in samples from Serum, Plasma, Cell supernatant

ELISA kit for Human HYOU1 (Hypoxia Up Regulated 1)
E-EL-H0426 1 plate of 96 wells
EUR 534
Description: A sandwich ELISA kit for quantitative measurement of Human HYOU1 (Hypoxia Up Regulated 1) in samples from Serum, Plasma, Cell supernatant

ELISA kit for Human HYOU1 (Hypoxia Up Regulated 1)
ELK3096 1 plate of 96 wells
EUR 432
Description: A sandwich ELISA kit for detection of Hypoxia Up Regulated 1 from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

ELISA kit for Rat HYOU1 (Hypoxia Up Regulated 1)
E-EL-R2533 1 plate of 96 wells
EUR 534
Description: A sandwich ELISA kit for quantitative measurement of Rat HYOU1 (Hypoxia Up Regulated 1) in samples from Serum, Plasma, Cell supernatant

Maximo H-SPlus Taq DNA Polymerase (for Hot-Start PCR)
S400 200 units
EUR 72

Maximo H-SPlus Taq DNA Polymerase (for Hot-Start PCR)
S410 1000 units
EUR 193

Accuris Hot Start Taq Mastermix
PR1001-HS-S 1 PC
EUR 69.79

Fire Start? PCR Mix-Dye
M1142-200
EUR 376

START domain containing 7 Antibody
DF13450 100ul
EUR 350

Exo-Flow 2.0 Basic Kit without antibody (Streptavidin beads + reagents) - for Serum or Plasma
EXOFLOW2-BASICA-SP 30 rxn
EUR 1001

Exo-Flow 2.0 Basic Kit without antibody (Streptavidin beads + reagents) - for Tissue Culture Media
EXOFLOW2-BASICA-TC 30 rxn
EUR 1001

PinPoint-HR System for Platform Cell Line Generation & Retargeting (includes PIN400A-1, PIN200A-1, PIN510A-1, & PIN600A-1)
PIN400A-KIT 1 Kit
EUR 2798

Frit Kit
FRIT-KIT 1each
EUR 124
Description: Kit to create frits in capillaries. Includes formamide, Kasil-1, Kasil-1624 and a cleaving tool.

TuMinute PCR Clean-Up Kit
P-1005 100 Samples
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Description: reagents widely cited

Human Proteinuria(UP)ELISA Kit
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EUR 289

Human Proteinuria(UP)ELISA Kit
GA-E1100HM-96T 96T
EUR 466

Guinea Pig UP ELISA Kit
EGU0018 96Tests
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Human Proteinuria,UP ELISA Kit
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EUR 440
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Up Plus RNA Extraction Kit
20-abx098093
  • EUR 370.00
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  • 100 rxns
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Human Proteinuria(UP)ELISA Kit
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Human Proteinuria,UP ELISA Kit
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Human Proteinuria,UP ELISA Kit
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PinPoint-FC System for Platform Cell Line Generation & Retargeting (includes PIN300A-1, FC200PA-1, PIN200A-1, PIN510A-1, & PIN600A-1)
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Custom Production of Monoclonal antibodies (immunization, ELISA Kit, fusion, screening, Cloning of up to 3 clones)
MAB-1 1
EUR 10403

Column Packing Kit
PACK-KIT 1pack
EUR 1035
Description: Column packing kit for pressure cells. Includes: HPREG regulator, TBNG10 tubing, CAP-75 capillary, and STRB5X2 stir bar.

Custom purification of IgY from up to 6 egg yolks
IGY-1 1
EUR 651

Custom purification of IgY from up to 12 egg yolks
IGY-2 1
EUR 895

ACTaq? Hot-Start DNA Polymerase, 1000U
E3500-1000U
EUR 446

ACTaq? Hot-Start DNA Polymerase, 250U
E3500-250U
EUR 170

amfiSure Hot-Start Taq DNA Polymerase
P0322-010 100 units
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amfiSure Hot-Start Taq DNA Polymerase
P0322-025 250 units
EUR 228

Cheetah Hot Start Taq DNA Polymerase
29050 500EU
EUR 296
Description: Minimum order quantity: 1 unit of 500EU

Accuris Hot Start Taq Master Mix
PR1001-HS-1000 1 PC
EUR 490.87

Accuris Hot Start Taq Master Mix
PR1001-HS-200 1 PC
EUR 169.47

PCR Mycoplasma Detection Kit
M034-Kit Kit
EUR 266

ELISA kit for Chicken Rudimental Bovine Serum albumin (BSA) check-up
KTE30161-48T 48T
EUR 354
Description: Quantitative sandwich ELISA for measuring Chicken Rudimental Bovine Serum albumin (BSA) check-up in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
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