gentaur.com

Novel Reagent Using Spectrophotometry

Sensitive detection of iron (II) sulfate with a novel reagent using spectrophotometry

In this study, a novel reagent was developed for sensitive detection of iron (II) sulfate, spectrophotometrically. A novel thio-anthraquinone derivative, 1-(Dodecylthio)anthracene-9,10-dione (3), was synthesized from the chemical reaction of 1-Chloroanthraquinone (1) and 1-Dodecanethiol (2) by an original reaction method and was used in the preparation of the novel reagent called Catal’s reagent.
  • A synthesized thio-anthraquinone analogue (3) was purified by column chromatography, and its chemical structure was characterized by spectroscopic methods such as Fourier-transform infrared spectroscopy (FT-IR), nuclear magnetic resonance (NMR) spectroscopy, mass spectrometry (MS), and ultraviolet (UV)-visible spectrophotometry.
  • The chemical and molecular structure of the developed thio-antraquinone derivative (3) was illuminated using computational methods with the GaussView5 and Gaussian09 programs. Various solvents including ethanol, methanol, and acetonitrile were examined in the preparation of the reagent. A concentration range from 0.2 mg mL-1 up to 10 mg mL-1 of iron (II) sulfate heptahydrate solution in distilled water was prepared. The absorption spectra of Catal’s reagent (0.816 mM) showed three peaks between 185 nm-700 nm of wavelength.
  • However, after the reaction with H2O2 and the 30 mM trisodium citrate dihydrate mixture in the presence of an iron sulfate (II) solution, a single peak was observed, producing a stable and reddish/brownish homogenous solution (λ max = 304 nm).
  • The following concentrations of iron (II) sulfate heptahydrate was examined using developed protocol and the reagent, and the concentrations were measured spectrophotometrically at 304 nm, 0.2-1 mg mL-1. Absorbances of reaction mixtures of iron (II) sulfate remained stable up to 48 h.
  • The results indicated that the novel Catal’s reagent can be used for sensitive spectrophotometric detection of iron (II) sulfate in aqueous solutions.
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gentaur

CD20 antibody (PE)

61R-1220 100 ug
EUR 528
Description: Rat monoclonal CD20 antibody (PE)

CD20 antibody (PE)

61R-CD20bHUPE 100 tests
EUR 379.2
Description: Mouse monoclonal CD20 antibody (PE)

CD103 / CD22 / CD20 Antibody (FITC, PE, PerCP)

abx200637-50tests 50 tests
EUR 927.6

human CD20/MS4A1 linear peptide (165-184, 20-aa, extracellular domain) broad reactivity with CD20-specific antibodies

CD20-165-P 100 ug
EUR 270

anti- SR-BI antibody

FNab08215 100µg
EUR 658.5
Description: Antibody raised against SR-BI

NATtrol Zika Virus Stock (Qualitative) (1 mL)

TEST 1 mL
EUR 1327.97
Description: Please contact Gentaur in order to receive the datasheet of the product.

Fluorescein Isothiocyanate (PE) Monoclonal Antibody (Pan-species), PE

4-MAO843Ge21-PE
  • EUR 356.40
  • EUR 3184.80
  • EUR 903.60
  • EUR 447.60
  • EUR 235.20
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Mouse monoclonal antibody against Pan-species Fluorescein Isothiocyanate (PE). This antibody is labeled with PE.

B-Lymphocyte Antigen CD20 (CD20) Antibody (PE)

abx139197-100tests 100 tests
EUR 577.2

B-Lymphocyte Antigen CD20 (CD20) Antibody (PE)

abx133981-100Assays 100 Assays
EUR 594

B-Lymphocyte Antigen CD20 (CD20) Antibody (PE)

abx133981-50Assays 50 Assays
EUR 477.6

B-Lymphocyte Antigen CD20 (CD20) Antibody (PE)

abx200174-100tests 100 tests
EUR 543.6

CD20 antibody (PE-CY7)

61R-CD20bHUPEC7 100 tests
EUR 535.2
Description: Mouse monoclonal CD20 antibody (PE-CY7)

Anti-Hu CD20 PE

1P-638-T025 25 tests
EUR 168

Anti-Hu CD20 PE

1P-638-T100 100 tests
EUR 288

CD20 antibody (FITC)

61R-CD20bHUFT 100 tests
EUR 327.6
Description: Mouse monoclonal CD20 antibody (FITC)

CD20 Antibody (FITC)

abx139195-100tests 100 tests
EUR 510

CD20 Antibody (FITC)

abx139196-100tests 100 tests
EUR 510

Humanized (chimeric) Anti-Human CD20/MS4A1 IgG (rituximab biosimilar), pure

CD20-23-M 100 ul
EUR 578.4

B-Lymphocyte Antigen CD20 (CD20) Antibody (PE-Cy5)

abx133982-100Assays 100 Assays
EUR 594

B-Lymphocyte Antigen CD20 (CD20) Antibody (PE-Cy5)

abx133982-50Assays 50 Assays
EUR 477.6

Human CD20/MS4A1 control/blocking peptide (EC-domain, rituximab binding domain)

CD20-22-P 100 ug
EUR 196.8

Asprosin Polyclonal Antibody (Human), PE

4-PAA332Hu01-PE
  • EUR 444.00
  • EUR 4437.60
  • EUR 1216.80
  • EUR 572.40
  • EUR 272.40
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Asprosin. This antibody is labeled with PE.

EZ-DNA Reagents

BS8202 100preps
EUR 105.67

EZ-DNA Reagents

SK8201 100preps
EUR 112.2

Ecl Detection Reagents

RPN2105 EACH
EUR 611.04

Ecl Detection Reagents

RPN3004 EACH
EUR 489.06

B-Lymphocyte Antigen CD20 (CD20) Antibody (FITC)

abx413278-01mg 0.1 mg
EUR 610.8

Recombinant (HEK cells) human CD20/MS4A1 (141-184 aa) hFc- fusion Protein

CD20-146-R 10 ug
EUR 489.6

Fluorescein Isothiocyanate (FITC) Monoclonal Antibody (Pan-species), FITC

4-MAO843Ge21-FITC
  • EUR 356.40
  • EUR 3184.80
  • EUR 903.60
  • EUR 447.60
  • EUR 235.20
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Mouse monoclonal antibody against Pan-species Fluorescein Isothiocyanate (FITC). This antibody is labeled with FITC.

Vinculin (VCL) Polyclonal Antibody (Human), PE

4-CAB839Hu01-PE
  • EUR 325.20
  • EUR 2736.00
  • EUR 792.00
  • EUR 403.20
  • EUR 220.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Vinculin (VCL). This antibody is labeled with PE.

Titin (TTN) Polyclonal Antibody (Human), PE

4-PAB667Hu01-PE
  • EUR 344.40
  • EUR 3012.00
  • EUR 860.40
  • EUR 430.80
  • EUR 230.40
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Titin (TTN). This antibody is labeled with PE.

Melanotransferrin (MFI2) Polyclonal Antibody (Human), PE

4-PAB677Hu01-PE
  • EUR 344.40
  • EUR 3012.00
  • EUR 860.40
  • EUR 430.80
  • EUR 230.40
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Melanotransferrin (MFI2). This antibody is labeled with PE.

Cholesterol (CH) Polyclonal Antibody (General), PE

4-PAB701Ge01-PE
  • EUR 339.60
  • EUR 2942.40
  • EUR 843.60
  • EUR 423.60
  • EUR 226.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against General Cholesterol (CH). This antibody is labeled with PE.

Glycocalicin (GC) Polyclonal Antibody (Human), PE

4-PAB710Hu01-PE
  • EUR 344.40
  • EUR 3012.00
  • EUR 860.40
  • EUR 430.80
  • EUR 230.40
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Glycocalicin (GC). This antibody is labeled with PE.

Fascin (FSCN) Polyclonal Antibody (Human), PE

4-PAB757Hu01-PE
  • EUR 344.40
  • EUR 3012.00
  • EUR 860.40
  • EUR 430.80
  • EUR 230.40
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Fascin (FSCN). This antibody is labeled with PE.

Fascin (FSCN) Polyclonal Antibody (Mouse), PE

4-PAB757Mu01-PE
  • EUR 350.40
  • EUR 3098.40
  • EUR 882.00
  • EUR 439.20
  • EUR 232.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Fascin (FSCN). This antibody is labeled with PE.

Neprilysin (NEP) Polyclonal Antibody (Mouse), PE

4-PAB785Mu01-PE
  • EUR 350.40
  • EUR 3098.40
  • EUR 882.00
  • EUR 439.20
  • EUR 232.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Neprilysin (NEP). This antibody is labeled with PE.

Neprilysin (NEP) Polyclonal Antibody (Rat), PE

4-PAB785Ra01-PE
  • EUR 362.40
  • EUR 3271.20
  • EUR 925.20
  • EUR 456.00
  • EUR 237.60
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Rat Neprilysin (NEP). This antibody is labeled with PE.

Neprilysin (NEP) Polyclonal Antibody (Rat), PE

4-PAB785Ra02-PE
  • EUR 362.40
  • EUR 3271.20
  • EUR 925.20
  • EUR 456.00
  • EUR 237.60
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Rat Neprilysin (NEP). This antibody is labeled with PE.

Arginine (Arg) Polyclonal Antibody (General), PE

4-PAB938Ge01-PE
  • EUR 338.40
  • EUR 2925.60
  • EUR 838.80
  • EUR 421.20
  • EUR 226.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against General Arginine (Arg). This antibody is labeled with PE.

Epiregulin (EREG) Polyclonal Antibody (Rat), PE

4-PAB945Ra01-PE
  • EUR 362.40
  • EUR 3271.20
  • EUR 925.20
  • EUR 456.00
  • EUR 237.60
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Rat Epiregulin (EREG). This antibody is labeled with PE.

Acrosin (ACR) Polyclonal Antibody (Rat), PE

4-PAB947Ra01-PE
  • EUR 362.40
  • EUR 3271.20
  • EUR 925.20
  • EUR 456.00
  • EUR 237.60
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Rat Acrosin (ACR). This antibody is labeled with PE.

Hepcidin (Hepc) Polyclonal Antibody (Bovine), PE

4-PAB979Bo01-PE
  • EUR 385.20
  • EUR 3600.00
  • EUR 1008.00
  • EUR 489.60
  • EUR 247.20
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Bovine Hepcidin (Hepc). This antibody is labeled with PE.

Hepcidin (Hepc) Polyclonal Antibody (Human), PE

4-PAB979Hu01-PE
  • EUR 344.40
  • EUR 3012.00
  • EUR 860.40
  • EUR 430.80
  • EUR 230.40
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Hepcidin (Hepc). This antibody is labeled with PE.

Hepcidin (Hepc) Polyclonal Antibody (Mouse), PE

4-PAB979Mu01-PE
  • EUR 350.40
  • EUR 3098.40
  • EUR 882.00
  • EUR 439.20
  • EUR 232.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Hepcidin (Hepc). This antibody is labeled with PE.

Hepcidin (Hepc) Polyclonal Antibody (Pig), PE

4-PAB979Po01-PE
  • EUR 385.20
  • EUR 3600.00
  • EUR 1008.00
  • EUR 489.60
  • EUR 247.20
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Pig Hepcidin (Hepc). This antibody is labeled with PE.

Hepcidin (Hepc) Polyclonal Antibody (Rat), PE

4-PAB979Ra01-PE
  • EUR 362.40
  • EUR 3271.20
  • EUR 925.20
  • EUR 456.00
  • EUR 237.60
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Rat Hepcidin (Hepc). This antibody is labeled with PE.

Hemopexin (HPX) Polyclonal Antibody (Human), PE

4-PAB986Hu01-PE
  • EUR 313.20
  • EUR 2563.20
  • EUR 748.80
  • EUR 385.20
  • EUR 216.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Hemopexin (HPX). This antibody is labeled with PE.

Hemopexin (HPX) Polyclonal Antibody (Human), PE

4-PAB986Hu02-PE
  • EUR 313.20
  • EUR 2563.20
  • EUR 748.80
  • EUR 385.20
  • EUR 216.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Hemopexin (HPX). This antibody is labeled with PE.

Hemopexin (HPX) Polyclonal Antibody (Rat), PE

4-PAB986Ra01-PE
  • EUR 362.40
  • EUR 3271.20
  • EUR 925.20
  • EUR 456.00
  • EUR 237.60
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Rat Hemopexin (HPX). This antibody is labeled with PE.

Hemopexin (HPX) Polyclonal Antibody (Rat), PE

4-PAB986Ra02-PE
  • EUR 362.40
  • EUR 3271.20
  • EUR 925.20
  • EUR 456.00
  • EUR 237.60
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Rat Hemopexin (HPX). This antibody is labeled with PE.

Hemojuvelin (HJV) Polyclonal Antibody (Human), PE

4-PAB995Hu01-PE
  • EUR 344.40
  • EUR 3012.00
  • EUR 860.40
  • EUR 430.80
  • EUR 230.40
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Hemojuvelin (HJV). This antibody is labeled with PE.

Lipopolysaccharide (LPS) Polyclonal Antibody (General), PE

4-PAB526Ge01-PE
  • EUR 339.60
  • EUR 2942.40
  • EUR 843.60
  • EUR 423.60
  • EUR 226.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against General Lipopolysaccharide (LPS). This antibody is labeled with PE.

Palladin (PALLD) Polyclonal Antibody (Human), PE

4-PAB590Hu01-PE
  • EUR 344.40
  • EUR 3012.00
  • EUR 860.40
  • EUR 430.80
  • EUR 230.40
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Palladin (PALLD). This antibody is labeled with PE.

Neuraminidase (NEU) Polyclonal Antibody (Human), PE

4-PAB611Hu01-PE
  • EUR 344.40
  • EUR 3012.00
  • EUR 860.40
  • EUR 430.80
  • EUR 230.40
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Neuraminidase (NEU). This antibody is labeled with PE.

Myostatin (MSTN) Polyclonal Antibody (Bovine), PE

4-PAB653Bo01-PE
  • EUR 385.20
  • EUR 3600.00
  • EUR 1008.00
  • EUR 489.60
  • EUR 247.20
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Bovine Myostatin (MSTN). This antibody is labeled with PE.

Myostatin (MSTN) Polyclonal Antibody (Human), PE

4-PAB653Hu01-PE
  • EUR 344.40
  • EUR 3012.00
  • EUR 860.40
  • EUR 430.80
  • EUR 230.40
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Myostatin (MSTN). This antibody is labeled with PE.

Myostatin (MSTN) Polyclonal Antibody (Mouse), PE

4-PAB653Mu01-PE
  • EUR 350.40
  • EUR 3098.40
  • EUR 882.00
  • EUR 439.20
  • EUR 232.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Myostatin (MSTN). This antibody is labeled with PE.

Myostatin (MSTN) Polyclonal Antibody (Pig), PE

4-PAB653Po01-PE
  • EUR 385.20
  • EUR 3600.00
  • EUR 1008.00
  • EUR 489.60
  • EUR 247.20
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Pig Myostatin (MSTN). This antibody is labeled with PE.

Myostatin (MSTN) Polyclonal Antibody (Human), PE

4-PAB653Ra01-PE
  • EUR 362.40
  • EUR 3271.20
  • EUR 925.20
  • EUR 456.00
  • EUR 237.60
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Myostatin (MSTN). This antibody is labeled with PE.

Prekallikrein (PK) Polyclonal Antibody (Human), PE

4-PAB801Hu01-PE
  • EUR 344.40
  • EUR 3012.00
  • EUR 860.40
  • EUR 430.80
  • EUR 230.40
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Prekallikrein (PK). This antibody is labeled with PE.

Versican (VS) Polyclonal Antibody (Human), PE

4-PAB817Hu01-PE
  • EUR 344.40
  • EUR 3012.00
  • EUR 860.40
  • EUR 430.80
  • EUR 230.40
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Versican (VS). This antibody is labeled with PE.

Versican (VS) Polyclonal Antibody (Mouse), PE

4-PAB817Mu01-PE
  • EUR 350.40
  • EUR 3098.40
  • EUR 882.00
  • EUR 439.20
  • EUR 232.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Versican (VS). This antibody is labeled with PE.

Vinculin (VCL) Polyclonal Antibody (Human), PE

4-PAB839Hu01-PE
  • EUR 344.40
  • EUR 3012.00
  • EUR 860.40
  • EUR 430.80
  • EUR 230.40
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Vinculin (VCL). This antibody is labeled with PE.

Paxillin (Pax) Polyclonal Antibody (Human), PE

4-PAB844Hu01-PE
  • EUR 344.40
  • EUR 3012.00
  • EUR 860.40
  • EUR 430.80
  • EUR 230.40
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Paxillin (Pax). This antibody is labeled with PE.

Nitrotyrosine (NT) Polyclonal Antibody (General), PE

4-PAB863Ge01-PE
  • EUR 339.60
  • EUR 2942.40
  • EUR 843.60
  • EUR 423.60
  • EUR 226.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against General Nitrotyrosine (NT). This antibody is labeled with PE.

Aggrecan (AGC) Polyclonal Antibody (Bovine), PE

4-PAB908Bo01-PE
  • EUR 384.00
  • EUR 3582.00
  • EUR 1003.20
  • EUR 487.20
  • EUR 246.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Bovine Aggrecan (AGC). This antibody is labeled with PE.

Aggrecan (AGC) Polyclonal Antibody (Human), PE

4-PAB908Hu01-PE
  • EUR 344.40
  • EUR 3012.00
  • EUR 860.40
  • EUR 430.80
  • EUR 230.40
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Aggrecan (AGC). This antibody is labeled with PE.

Aggrecan (AGC) Polyclonal Antibody (Human), PE

4-PAB908Hu02-PE
  • EUR 344.40
  • EUR 3012.00
  • EUR 860.40
  • EUR 430.80
  • EUR 230.40
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Aggrecan (AGC). This antibody is labeled with PE.

Aggrecan (AGC) Polyclonal Antibody (Human), PE

4-PAB908Hu03-PE
  • EUR 344.40
  • EUR 3003.60
  • EUR 858.00
  • EUR 429.60
  • EUR 229.20
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Aggrecan (AGC). This antibody is labeled with PE.

Aggrecan (AGC) Polyclonal Antibody (Mouse), PE

4-PAB908Mu01-PE
  • EUR 350.40
  • EUR 3098.40
  • EUR 882.00
  • EUR 439.20
  • EUR 232.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Aggrecan (AGC). This antibody is labeled with PE.

Aggrecan (AGC) Polyclonal Antibody (Mouse), PE

4-PAB908Mu02-PE
  • EUR 350.40
  • EUR 3098.40
  • EUR 882.00
  • EUR 439.20
  • EUR 232.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Aggrecan (AGC). This antibody is labeled with PE.

Aggrecan (AGC) Polyclonal Antibody (Rat), PE

4-PAB908Ra01-PE
  • EUR 362.40
  • EUR 3271.20
  • EUR 925.20
  • EUR 456.00
  • EUR 237.60
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Rat Aggrecan (AGC). This antibody is labeled with PE.

Aggrecan (AGC) Polyclonal Antibody (Rat), PE

4-PAB908Ra02-PE
  • EUR 362.40
  • EUR 3271.20
  • EUR 925.20
  • EUR 456.00
  • EUR 237.60
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Rat Aggrecan (AGC). This antibody is labeled with PE.

Hemoglobin (HB) Polyclonal Antibody (Bovine), PE

4-PAB409Bo01-PE
  • EUR 321.60
  • EUR 2683.20
  • EUR 778.80
  • EUR 397.20
  • EUR 219.60
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Bovine Hemoglobin (HB). This antibody is labeled with PE.

Hemoglobin (HB) Polyclonal Antibody (Mouse), PE

4-PAB409Mu01-PE
  • EUR 237.60
  • EUR 1491.60
  • EUR 480.00
  • EUR 278.40
  • EUR 183.60
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Hemoglobin (HB). This antibody is labeled with PE.

Hemoglobin (HB) Polyclonal Antibody (Pig), PE

4-PAB409Po01-PE
  • EUR 321.60
  • EUR 2683.20
  • EUR 778.80
  • EUR 397.20
  • EUR 219.60
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Pig Hemoglobin (HB). This antibody is labeled with PE.

Hemoglobin (HB) Polyclonal Antibody (Rabbit), PE

4-PAB409Rb51-PE
  • EUR 241.20
  • EUR 1543.20
  • EUR 493.20
  • EUR 283.20
  • EUR 186.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Guinea polyclonal antibody against Rabbit Hemoglobin (HB). This antibody is labeled with PE.

Acetylcholinesterase (AchE) Polyclonal Antibody (Human), PE

4-PAB447Hu01-PE
  • EUR 344.40
  • EUR 3012.00
  • EUR 860.40
  • EUR 430.80
  • EUR 230.40
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Acetylcholinesterase (AchE). This antibody is labeled with PE.

Acetylcholinesterase (AchE) Polyclonal Antibody (Mouse), PE

4-PAB447Mu01-PE
  • EUR 350.40
  • EUR 3098.40
  • EUR 882.00
  • EUR 439.20
  • EUR 232.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Acetylcholinesterase (AchE). This antibody is labeled with PE.

Acetylcholinesterase (AchE) Polyclonal Antibody (Mouse), PE

4-PAB447Mu02-PE
  • EUR 350.40
  • EUR 3098.40
  • EUR 882.00
  • EUR 439.20
  • EUR 232.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Acetylcholinesterase (AchE). This antibody is labeled with PE.

Acetylcholinesterase (AchE) Polyclonal Antibody (Rat), PE

4-PAB447Ra02-PE
  • EUR 362.40
  • EUR 3271.20
  • EUR 925.20
  • EUR 456.00
  • EUR 237.60
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Rat Acetylcholinesterase (AchE). This antibody is labeled with PE.

Ovalbumin (OVA) Polyclonal Antibody (Chicken), PE

4-PAB459Ge01-PE
  • EUR 338.40
  • EUR 2925.60
  • EUR 838.80
  • EUR 421.20
  • EUR 226.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Chicken Ovalbumin (OVA). This antibody is labeled with PE.

Fibromodulin (FMOD) Polyclonal Antibody (Human), PE

4-PAB494Hu01-PE
  • EUR 344.40
  • EUR 3012.00
  • EUR 860.40
  • EUR 430.80
  • EUR 230.40
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Fibromodulin (FMOD). This antibody is labeled with PE.

Fibromodulin (FMOD) Polyclonal Antibody (Rat), PE

4-PAB494Ra01-PE
  • EUR 362.40
  • EUR 3271.20
  • EUR 925.20
  • EUR 456.00
  • EUR 237.60
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Rat Fibromodulin (FMOD). This antibody is labeled with PE.

Lumican (LUM) Polyclonal Antibody (Human), PE

4-PAB496Hu01-PE
  • EUR 344.40
  • EUR 3012.00
  • EUR 860.40
  • EUR 430.80
  • EUR 230.40
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Lumican (LUM). This antibody is labeled with PE.

Lumican (LUM) Polyclonal Antibody (Mouse), PE

4-PAB496Mu01-PE
  • EUR 350.40
  • EUR 3098.40
  • EUR 882.00
  • EUR 439.20
  • EUR 232.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Lumican (LUM). This antibody is labeled with PE.

Dystrophin (DMD) Polyclonal Antibody (Human), PE

4-PAB503Hu01-PE
  • EUR 344.40
  • EUR 3012.00
  • EUR 860.40
  • EUR 430.80
  • EUR 230.40
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Dystrophin (DMD). This antibody is labeled with PE.

Dystrophin (DMD) Polyclonal Antibody (Mouse), PE

4-PAB503Mu01-PE
  • EUR 350.40
  • EUR 3098.40
  • EUR 882.00
  • EUR 439.20
  • EUR 232.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Dystrophin (DMD). This antibody is labeled with PE.

 

Engineering caspase 7 as an affinity reagent to capture proteolytic products

Many proteases recognize their substrates with high specificities, with this in mind, it should theoretically be possible to utilize the substrate binding cleft of a protease as a scaffold to engineer an affinity reagent. In this study, we sought to develop reagents that would differentiate between substrates and products of proteolysis, based on a caspase 7 scaffold. Firstly, we engineered a form of caspase 7 that can undergo conversion to a substrate binding conformation without catalysis. Seeking to generate a product-only trap, we further engineered this construct by incorporating mutations that compensate for the generation of a negative charge in the neo C terminus of a newly generated product.
  • This was accomplished with only three substitutions within the substrate binding cleft. Moreover, the affinity of the product trap for peptides was comparable to the affinity of caspase 7 to parental substrates.
  • Finally, generation of a hybrid fluorescent protein with the product trap provided a reagent that specifically recognized apoptotic cells and highlights the versatility of such an approach in developing affinity and imaging agents for a variety of cysteine and serine proteases.

Leveraging nature’s biomolecular designs in next-generation protein sequencing reagent development

Next-generation approaches for protein sequencing are now emerging that could have the potential to revolutionize the field in proteomics. One such sequencing method involves fluorescence-based imaging of immobilized peptides in which the N-terminal amino acid of a polypeptide is readout sequentially by a series of fluorescently labeled biomolecules.

When selectively bound to a specific N-terminal amino acid, the NAAB (N-terminal amino acid binder) affinity reagent identifies the amino acid through its associated fluorescence tag. A key technical challenge in implementing this fluoro-sequencing approach is the need to develop NAAB affinity reagents with the high affinity and selectivity for specific N-terminal amino acids required for this biotechnology application.

One approach to develop such a NAAB affinity reagent is to leverage naturally occurring biomolecules that bind amino acids and/or peptides. Here, we describe several candidate biomolecules that could be considered for this purpose and discuss the potential for developability of each.

Key points • Next-generation sequencing methods are emerging that could revolutionize proteomics. • Sequential readout of N-terminal amino acids by fluorescent-tagged affinity reagents. • Native peptide/amino acid binders can be engineered into affinity reagents. • Protein size and structure contribute to feasibility of reagent developability.

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