August 26, 2020
Cross-Couplings between Alkyl Halides and Grignard Reagents
Cobalt-Catalyzed Cross-Couplings between Alkyl Halides and Grignard Reagents
ConspectusMetal-catalyzed cross-couplings have emerged as necessary devices for the event of C-C bonds. The identification of setting pleasant catalytic packages along with huge substrate scope made these cross-couplings key reactions to entry useful molecules ranging from provides, agrochemicals to vigorous pharmaceutical parts.
They have been an increasing number of built-in in retrosynthetic plans, allowing shorter and genuine route enchancment. Palladium-catalyzed cross-couplings nonetheless largely rule the sphere, with essentially the most well-liked reactions in industrial processes being the Suzuki and Sonogashira couplings.
Nonetheless, the intensive use of palladium complexes raises plenty of points equivalent to limited belongings, excessiveprice, environmental affect, and frequent wantfor fashionable ligands. As a consequence, utilizing nonprecious and low price metallic catalysts has appeared as a model new horizon in cross-coupling enchancment.
Over the previous three a few years, a rising curiosity has thus been devoted to Fe-, Co-, Cu-, or Ni-catalyzed cross-couplings. Their pure abundance makes them cost-effective, allowing the conception of additional sustainable and cheaper chemical processes, significantly for large-scale manufacturing of vigorous molecules.
- Together with these economical and environmental considerations, the 3d metallic catalysts moreover exhibit complementary reactivity with palladium complexes, facilitating utilizing alkyl halide companions attributable to the decrease of β-elimination side reactions.
- Particularly, by using cobalt catalysts, fairly just a few cross-couplings between alkyl halides and organometallics have been described.
- Nonetheless, cobalt catalysis nonetheless stays far behind palladium catalysis with regards to fame and functions, and the enlargement of the substrate scope along with the occasion of easy and durable catalytic packages stays an needed drawback.
- In 2012, our group entered the cobalt catalysis space by rising a cobalt-catalyzed cross-coupling between C-bromo glycosides and Grignard reagents. The generality of the coupling allowed the preparation of a wide range of useful C-aryl and C-vinyl glycoside establishing blocks.
- We then centered on the functionalization of saturated N-heterocycles, and various halo-azetidines, -pyrrolidines, and -piperidines had been effectively reacted with aryl and alkenyl Grignard reagents beneath cobalt catalysis.
- With the goal of creating prepared useful α-aryl amides, a cobalt-catalyzed cross-coupling utilized to α-bromo amides was studied after which extended to α-bromo lactams.
- These days, we moreover reported an setting pleasant and regular cross-coupling involving cyclopropyl- and cyclobutyl-magnesium bromides. This technique permits the alkylation of functionalized small strained rings by a wide range of main and secondary alkyl halides.
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High-density Lipoprotein Cholesterol -HDL-C- Colorimetric Assay Kit -Double reagents- |
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Comparative analysis between a medical system and regent dipsticks in measuring pH
Targets: Urinary pH is a decisive take into consideration plenty of pathologies, there by an informative marker employed in remedy selections. Although extensively used, the urinary pH dipstick check out won’t be sufficiently right or actual for scientific selections and additional sturdy methodologies must be considered. On this analysis, we consider pH measurements when using aportable medical system and completely completely different dipstick exams.
Provides and techniques:Four pH dipstick producers and a Lit-Administration® pH Meter had been examined using enterprise buffer choices with seven distinct pH values representing the physiological differ in urine (4.66; 5.0;5.5; 6.0; 7.0; 7.5; 8.0). A statistical analysis was carried out to guage the correlation of measured versus precise values, together with validity measures as resolution,precision and accuracy.
Outcomes: Validity measures stated the prevalence of the moveable pH meter, with a decreased dispersion of data and additional precise values. Furthermore, correlation analysis reveal that the pH values obtained with the pH meter had been the closest to the buffers’ precise pH values.
Conclusion: The detailed comparative analysis launched proper right here reveal the prevalence of a conveyable pH meter to plenty of of primarily essentially the most used dipstick producers inside the clinic.Lit-Administration® pH Meter represents a reliable completely different when a monitoring of urinary pH is required, as may happen in the middle of the screening of illnesses or remedy monitoringin the clinic, along with in the middle of the self-monitoringby the affected particular person beneath expert supervision at dwelling.
Objetivos: El pH urinario es un subject decisivo en varias patologías, en consecuencia, un marcador informativo empleado en las decisiones de tratamiento. Aunque ampliamente utilizada, la tira reactiva puede no ser lo suficientemente acurada o precisa para la toma de decisiones clínicas y otras metodologías más robustas deben ser consideradas.
En este estudio,comparamos las mediciones de pH cuando usamos un dispositivo médico portátil y diferentes tiras reactivas.
MATERIALES Y MÉTODOS: Se probaron cuatro marcas de tiras reactives de pH y un medidor de pH, Lit-Administration® pH Meter, utilizando soluciones tampón comerciales con siete valores de pH distintos que representan el rango fisiológico de pH en orina (4.66; 5.0; 5.5; 6.0;7.0; 7.5; 8.0). Se realizó un análisis estadístico para evaluar la correlación de los valores medidos versus los reales, junto con las medidas de validez como resolución,precisión y exactitud.
Resultados: Las medidas de validez indicaron la superioridad del medidor de pH portátil, con una reducción de la dispersión de los datos y valores más exactos. Además, el análisis de correlación demuestra que los valores de pH obtenidos con el medidor de pH fueron los más cercanos a los valores de pH reales de los tampones.
CONCLUSIÓN: El estudio comparativo detallado presentado aquí revela la superioridad de un medidor portátil de pH frente algunas de las marcas comerciales de tiras reactivas más ampliamente usadas en las práctica clínica. El Lit-Administration® pH Meter representa una alternativa fiable cuando se necesita un monitoreo del pH urinario, como puede suceder durante la detección de enfermedades o la monitorización del tratamiento a nivel clínico, así como durante el autocontrol domiciliario del paciente, bajo supervisión profesional.
Key phrases: Cálculos renales; Dispositivo médico; Kidney stones; Lithiasis; Litiasis; Medical system; Medidor de pH; Urinary pH; Urolithiasis; Urolitiasis; pH meter; pH urinario.
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PeliKine tool set, additional reagents |
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iN-fect™ In vitro transfection reagents |
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Description: Transfection |
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Pre-Titrated Reagents for Human EGF ELISA |
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Leeporter Assay Reagent- 1000 Test |
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Leeporter Assay Reagent- 1000 Test |
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Liposomal and Non-Liposomal Transfection Reagents |
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Liposomal and Non-Liposomal Transfection Reagents |
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Pre-Titrated Reagents for Human IL-8 ELISA |
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Pre-Titrated Reagents for Human IL-8 ELISA |
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Pre-Titrated Reagents for Human MCP-1 ELISA |
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| MBS590075-10Plates | MyBiosource | 10Plates | EUR 710 |
Pre-Titrated Reagents for Human MCP-1 ELISA |
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Pre-Titrated Reagents for Human MCP-1 ELISA |
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Pre-Titrated Reagents for Human MCP-3 ELISA |
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Pre-Titrated Reagents for Human MCP-3 ELISA |
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Pre-Titrated Reagents for Human MCP-3 ELISA |
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Pre-Titrated Reagents for Human IL-16 ELISA |
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Pre-Titrated Reagents for Human IL-16 ELISA |
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Pre-Titrated Reagents for Human IL-16 ELISA |
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Pre-Titrated Reagents for Human IL-18 ELISA |
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Pre-Titrated Reagents for Human IL-18 ELISA |
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Pre-Titrated Reagents for Human IL-18 ELISA |
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Pre-Titrated Reagents for Human GM-CSF ELISA |
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Pre-Titrated Reagents for Human GM-CSF ELISA |
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Pre-Titrated Reagents for Human GM-CSF ELISA |
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Pre-Titrated Reagents for Human TNF-alpha ELISA |
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Pre-Titrated Reagents for Human TNF-alpha ELISA |
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| MBS590078-5Plates | MyBiosource | 5Plates | EUR 520 |
Pre-Titrated Reagents for Human TNF-alpha ELISA |
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| MBS590078-5x10Plates | MyBiosource | 5x10Plates | EUR 3270 |
Barritt Reagent A (for VP test) |
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| R029-100ML | EWC Diagnostics | 1 unit | EUR 6.48 |
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Description: Barritt Reagent A (for VP test) |
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Barritt Reagent B (for VP test) |
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| R030-100ML | EWC Diagnostics | 1 unit | EUR 2.89 |
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Description: Barritt Reagent B (for VP test) |
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RANGE 13 - Ventilated cabinet for reagents and toxic products - 250 L |
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| MF4V | Ecosafe Sa | each | EUR 2377.76 |
RANGE 13 - Cabinet for reagents and toxic products (without fan) - 250 L |
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| MF4SV. | Ecosafe Sa | each | EUR 2158.24 |
ImmunoGold labeling reagents for (TEM) - Unconjugated gold colloid (GC), 5nm |
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| 22716-100 | Polysciences Europe GmbH | 100ml | EUR 1037.88 |
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Description: 7732-18-5 |
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ImmunoGold labeling reagents for (TEM) - Unconjugated gold colloid (GC), 10nm |
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| 22717-100 | Polysciences Europe GmbH | 100ml | EUR 1022.76 |
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Description: 7732-18-5 |
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ImmunoGold labeling reagents for (TEM) - Unconjugated gold colloid (GC), 15nm |
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| 22718-100 | Polysciences Europe GmbH | 100ml | EUR 1023.84 |
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Description: 7732-18-5 |
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ImmunoGold labeling reagents for (SEM) - Unconjugated gold colloid (GC) 20nm |
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| 22719-100 | Polysciences Europe GmbH | 100ml | EUR 1036.8 |
COMBI IC Reagent: Mouse anti CD45 (FITC) and Mouse anti CD14 (PE) |
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| MBS570370-50Tests | MyBiosource | 50Tests | EUR 450 |
COMBI IC Reagent: Mouse anti CD45 (FITC) and Mouse anti CD14 (PE) |
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| MBS570370-5x50Tests | MyBiosource | 5x50Tests | EUR 1885 |
Amersham ECL Western Blotting Detection Reagents for 4000cm2 membrane - EACH |
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Amersham ECL Western Blotting Detection Reagents for 1000cm2 membrane - EACH |
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| RPN2109 | Scientific Laboratory Supplies | EACH | EUR 211.95 |
Amersham ECL Western Blotting Detection Reagents for 6000cm2 membrane - 1KIT |
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| RPN2134 | Scientific Laboratory Supplies | 1KIT | EUR 643.95 |
Amersham ECL Western Blotting Detection Reagents for 2000cm2 membrane - EACH |
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| RPN2209 | Scientific Laboratory Supplies | EACH | EUR 328.05 |
T7 gRNA SmartNuclease Synthesis Kit (includes CAS510A-1 & T7 IVT synthesis reagents) |
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| CAS510A-KIT | SBI | 1 Kit | EUR 724 |
Low-density Lipoprotein Cholesterol -LDL-C- Colorimetric Assay Kit -Double Reagents- |
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| E-BC-K205-96T | Elabscience Biotech | 96T | EUR 150 |
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Description: Quantitative |
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Low-density Lipoprotein Cholesterol -LDL-C- Colorimetric Assay Kit -Double Reagents- |
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| E-BC-K205-each | Elabscience Biotech | each | Ask for price |
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Description: Quantitative |
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Low-density Lipoprotein Cholesterol -LDL-C- Colorimetric Assay Kit -Double Reagents- |
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| E-BC-K206-S-100Assays | Elabscience Biotech | 100 Assays | EUR 240 |
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Description: Quantitative |
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Low-density Lipoprotein Cholesterol -LDL-C- Colorimetric Assay Kit -Double Reagents- |
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| E-BC-K206-S-each | Elabscience Biotech | each | Ask for price |
|
Description: Quantitative |
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High-density Lipoprotein Cholesterol -HDL-C- Colorimetric Assay Kit -Double reagents- |
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| E-BC-K221-96T | Elabscience Biotech | 96T | EUR 150 |
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Description: Quantitative |
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High-density Lipoprotein Cholesterol -HDL-C- Colorimetric Assay Kit -Double reagents- |
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| E-BC-K221-each | Elabscience Biotech | each | Ask for price |
|
Description: Quantitative |
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High-density Lipoprotein Cholesterol -HDL-C- Colorimetric Assay Kit -Double reagents- |
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| E-BC-K222-S-100Assays | Elabscience Biotech | 100 Assays | EUR 240 |
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Description: Quantitative |
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High-density Lipoprotein Cholesterol -HDL-C- Colorimetric Assay Kit -Double reagents- |
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| E-BC-K222-S-each | Elabscience Biotech | each | Ask for price |
|
Description: Quantitative |
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RPE REFERENCE STANDARD Reagent |
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| GWB-Q00331 | GenWay Biotech | 100 TESTS | Ask for price |
STREPTAVIDIN: RPE-Cy5.5 Reagent |
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| GWB-Q00339 | GenWay Biotech | 0.5 mg | Ask for price |
STREPTAVIDIN: RPE-Cy5.5 Reagent |
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| GWB-Q00340 | GenWay Biotech | 0.1 mg | Ask for price |
STREPTAVIDIN: RPE-Cy7 Reagent |
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| GWB-Q00341 | GenWay Biotech | 0.5 mg | Ask for price |
STREPTAVIDIN: RPE-Cy7 Reagent |
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| GWB-Q00342 | GenWay Biotech | 0.1 mg | Ask for price |
Reagents for the identification and qualitative measurement of anti-Zika NS1 antibodies. |
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| ELS61258 | The Native Antigen Company | 3 x 96 well plate kit | EUR 641.21 |
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Description: Zika Virus Total Antibody ELISA |
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Exo-Flow 2.0 Basic Kit without antibody (Streptavidin beads + reagents) - for Serum or Plasma |
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| EXOFLOW2-BASICA-SP | SBI | 30 rxn | EUR 1201.2 |
COMBI IC Reagent: Mouse anti Myeloperoxidase-C2 (FITC) and Mouse anti CD22 (PE) |
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| MBS570348-1mL | MyBiosource | 1mL | EUR 615 |
COMBI IC Reagent: Mouse anti Myeloperoxidase-C2 (FITC) and Mouse anti CD22 (PE) |
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| MBS570348-5x1mL | MyBiosource | 5x1mL | EUR 2620 |
Exo-Flow 2.0 Basic Kit without antibody (Streptavidin beads + reagents) - for Tissue Culture Media |
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| EXOFLOW2-BASICA-TC | SBI | 30 rxn | EUR 1201.2 |
cAMP Biotrak(TM) EIA; (Non-Acetylation Protocol; Lysis Reagents not Included); Cytiva; RPN2251 - EACH |
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| GERPN2251 | Scientific Laboratory Supplies | EACH | EUR 770.85 |
COMBI IC Reagent: Mouse anti Myeloperoxidase-C2 (FITC) and Mouse anti CD3 (PE) |
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| MBS570355-1mL | MyBiosource | 1mL | EUR 615 |
COMBI IC Reagent: Mouse anti Myeloperoxidase-C2 (FITC) and Mouse anti CD3 (PE) |
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| MBS570355-5x1mL | MyBiosource | 5x1mL | EUR 2620 |
STREPTAVIDIN: FITC Reagent |
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| GWB-Q00335 | GenWay Biotech | 1 mg | Ask for price |
QUANTUM FITC-5 MESF Reagent |
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| GWB-Q00318 | GenWay Biotech | 20 TESTS | Ask for price |
QUANTUM FITC-5 MESF Reagent |
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| GWB-Q00319 | GenWay Biotech | 100 TESTS | Ask for price |
QUANTUM FITC-5 MESF Reagent |
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| GWB-Q00320 | GenWay Biotech | 280 TESTS | Ask for price |
OOSA09633-100T - RPE REFERENCE STANDARD Accessory Reagent |
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| OOSA09633-100T | Aviva Systems Biology | 100Tests | EUR 889 |
TruStrip WBV Tubulin protein quantitation & Western Blot Validation Kit (contains actin strips, primary antibodies and Western reagents 100 strips or 10 miniblot) |
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| WBTUB100-K | Alpha Diagnostics | 1 Kit | EUR 634.8 |
COMBI IC Reagent: IgG Negative Control (FITC) and IgG Negative Control (PE) |
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| MBS570400-1mL | MyBiosource | 1mL | EUR 370 |
COMBI IC Reagent: IgG Negative Control (FITC) and IgG Negative Control (PE) |
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| MBS570400-5x1mL | MyBiosource | 5x1mL | EUR 1525 |
COMBI IC Reagent: Mouse anti Lysozyme (FITC) and Mouse anti Lactoferrin (PE) |
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| MBS570350-1mL | MyBiosource | 1mL | EUR 615 |
COMBI IC Reagent: Mouse anti Lysozyme (FITC) and Mouse anti Lactoferrin (PE) |
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| MBS570350-5x1mL | MyBiosource | 5x1mL | EUR 2620 |
TruStrip WBV Human Beta Actin Protein Quantitation & Western Blot Validation Kit (contains actin strips, primary antibodies and Western reagents 100 strips or 10 miniblot) |
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| WBACT100-K | Alpha Diagnostics | 1 Kit | EUR 634.8 |
OOSA09643-280T - RPE-TR REFERENCE STANDARD Accessory Reagent |
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| OOSA09643-280T | Aviva Systems Biology | 280Tests | EUR 429 |
OOSA09640-280T - RPE-Cy5 REFERENCE STANDARD Accessory Reagent |
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| OOSA09640-280T | Aviva Systems Biology | 280Tests | EUR 429 |
QUANTUM FITC-5 MESF (PRE-MIXED) Reagent |
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| GWB-Q00321 | GenWay Biotech | 20 TESTS | Ask for price |
QUANTUM FITC-5 MESF (PRE-MIXED) Reagent |
|||
| GWB-Q00322 | GenWay Biotech | 100 TESTS | Ask for price |
COMBI IC Reagent: Mouse anti Myeloperoxidase-C2 (FITC) and Mouse anti Lactoferrin (PE) |
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| MBS570364-1mL | MyBiosource | 1mL | EUR 615 |
COMBI IC Reagent: Mouse anti Myeloperoxidase-C2 (FITC) and Mouse anti Lactoferrin (PE) |
|||
| MBS570364-5x1mL | MyBiosource | 5x1mL | EUR 2620 |
QUANTUM R-PE MESF MEDIUM LEVEL Reagent |
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| GWB-Q00324 | GenWay Biotech | 20 TESTS | Ask for price |
QUANTUM R-PE MESF MEDIUM LEVEL Reagent |
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| GWB-Q00325 | GenWay Biotech | 100 TESTS | Ask for price |
CD4 FITC - CD26 PE |
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| MBS396187-50Tests | MyBiosource | 50Tests | EUR 345 |
