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August 26, 2020

Commercially available transaction reagents 

By Danielle

Commercially out there transaction reagents and damaging management siRNA will not be inert

The transfection of artificial small interfering (si)RNA into cultured cells kinds the premise of research that use RNA interference (generally known as “gene knockdown”) to check the impression of lack of gene or protein expression on a organic pathway or course of. In these research, mock transfections (with transfection reagents alone), and the usage of artificial damaging management (apparently inert) siRNA are each important damaging controls.

This report reveals that three widely-used transfection reagents (X-tremeGENE™, HiPerFect, and Lipofectamine® 2000) and 5 commercially-available management siRNA (from Ambion, Sigma, Santa Cruz, Cell Signaling Know-how, and Qiagen) will not be inert in cell-culture research.

Each transfection reagents and management siRNA perturbed steady-state mRNA and protein ranges in major mouse lung fibroblasts and in NIH/3T3 cells (a widely-used mouse embryonic fibroblast cell-line), utilizing parts of the canonical reworking development factor-β signaling equipment as a mannequin system.

Moreover, transfection reagents and management siRNA decreased the viability and proliferation of each lung fibroblasts and NIH/3T3 cells. These information collectively present a cautionary word to investigators to fastidiously think about the impression of management interventions, reminiscent of mock transfections and management siRNA, in RNA interference research with artificial siRNA.

gentaur.com

gentaur.com

ECL SELECT WB DETECTION REAGENT
GERPN2235 EACH
EUR 331.2

Ecl Select Wb Detection Reagent Kit
RPN2235 EACH
EUR 375.6

Detection Reagent A
abx296004-120ul 120 ul
EUR 385.2

GST Detection Reagent, 1EA
C035-1EA 1EA
EUR 218

ThioStar® Detection Reagent, 1EA
C012-1EA 1EA
EUR 212

ThioStar® Detection Reagent, 1EA
C036-1EA 1EA
EUR 687

ThioStar® Detection Reagent, 1EA
C048-1EA 1EA
EUR 242

ThioStar® Detection Reagent, 1EA
C053-1EA 1EA
EUR 242

Hemoglobin Detection Reagent, 20ML
C038-20ML 20ML
EUR 242

Fluorescent Detection Reagent, 5ML
C116-5ML 5ML
EUR 254

Detection Reagent Concentrate, 1ML
X041-1ML 1ML
EUR 109

QuantiFluo™ ALP Detection Reagent
QFALP-6mL 600
EUR 129
Description: Stabilized 4-methylumbelliferyl phosphate (MUP) liquid substrate reagent for detecting alkaline phosphatase ALP, e.g. in ELISA. This single reagent produces a highly fluorescent product (360/450 nm) when dephosphorylated by ALP. The fluorescent product can also be visualized under a UV light. Key Features: Stable single reagent. Highly sensitive fluorescence detection. Can be visualized under a UV light. Method: FL360/450nm. Samples: Biological. Species: All. Procedure: Assay takes 30 min. Kit size: 600 tests.

QuantiFluo™ HRP Detection Reagent
QFHRP-25mL 500
EUR 249
Description: Stabilized 10-Acetyl-3,7-dihydroxyphenoxazine (also called Ampliflu Red) liquid substrate reagent for detecting horseradish peroxidase HRP activity, e.g. in ELISA. This reagent produces a highly fluorescent (530/585nm), pink-colored product upon reaction with a peroxidase. The fluorescent product can also be visualized under a light source (510-550 nm). Key Features: For both absorbance or fluorescence detection. Highly sensitive. Convenient and fast. Method: OD570nm, FL530/585nm. Samples: Biological. Species: All. Procedure: Assay takes 30 min. Kit size: 500 tests.

Protein Detection Reagent unit: 100 ml
FYP202-100ML 100ml Ask for price

QuantiChrom™ HRP Detection Reagent
DTMB-200 200
EUR 159
Description: A stabilized sensitive reagent for peroxidases, for use in ELISA, immunochromogenic chemistry and Western blot. Key Features: Highly sensitive. Detects as low as 7.6x10-8 U/well, or 7.7 pg/well horseradish peroxidase (HRP) in 96-well plate assays. Detects as low as 0.1 ppb Gliadin in ELISA. Improved stability and assay robustness. Method: OD450nm. Samples: peroxidases and HRP in ELISA. Species: All. Procedure: Assay takes Approx. 30 min. Kit size: 200 Tests. Detection limit: 76 nU/well, or 7.7 pg/well.

PdX™ Palladium Detection Reagent, 3ML
C017-3ML 3ML
EUR 545

SuperBrite? ECL Western Blot Substrate/Detection Kit
K824-50 each
EUR 229.2

High-Sensitive ECL Chemiluminescence Detection Kit (Ready-to-Use)
E412-01 2 × 50 ml
EUR 180

High-Sensitive ECL Chemiluminescence Detection Kit (Ready-to-Use)
E412-02 250 ml
EUR 393.6

T-Pro LumiFast Plus Chemiluminescence Detection Kit (ECL Kit)
JT96-K002M 250ml*2/Kit
EUR 244.8

T-Pro LumiFast Plus Chemiluminescence Detection Kit (ECL Kit)
JT96-K002S 100ml*2/Kit
EUR 182.4

T-Pro LumiLong Plus Chemiluminescence Detection Kit (ECL Kit)
JT96-K004M 250ml*2/Kit
EUR 286.8

T-Pro LumiLong Plus Chemiluminescence Detection Kit (ECL Kit)
JT96-K004S 100ml*2/Kit
EUR 204

Amersham ECL Western Blotting Systemor detection for 1000 cm2 membrane
RPN2108 EACH
EUR 326.4

EZ-DNA Reagents
BS8202 100preps
EUR 105.67

EZ-DNA Reagents
SK8201 100preps
EUR 112.2

REAGENTS HIGH RANGE AMMONIA
HI73325 PK25
EUR 49.2

High Range Ammonia Reagents
HI9373301 EACH
EUR 116.4

CryoProtX Mix Reagents 1.5 mL
M-MDSR-61 1.5 ml ml
EUR 37
Description: CryoProtX Mix Reagents 1.5 mL

Alkphos Direct Labelling Reagents
RPN3680 EACH
EUR 813.6

Set of 10 Biolipidure Reagents
Biolipidure-set 10mLx10
EUR 1820.4
Description: Set of 10 Biolipidure Reagents, whose applications include Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement

ECL Start Reagent 2000cm2
RPN3243 EACH
EUR 138

ECL Start Reagent 4000cm2
RPN3244 EACH
EUR 222

ECL Start Reagent 200cm2
RPN3245 EACH
EUR 26.4

CryoProtX Mix Eco Reagents 1.5 mL
M-MDSR-61-ECO 1.5 ml ml
EUR 37
Description: CryoProtX Mix Eco Reagents 1.5 mL

Free Chlorine Reagents 0-5mg/L
WAT1028 EACH
EUR 44.4

NGAL (Detection Ab)
abx019242-100ug 100 ug
EUR 777.6

SEB Detection Kit
6030 1 kit
EUR 663.78
Description: SEB Detection Kit

TLR Detection Set
PSI-1806 1 Set
EUR 1627.8
Description: Toll-like receptors (TLRs) are evolutionarily conserved pattern-recognition molecules resembling the toll proteins that mediate antimicrobial responses in Drosophila. These proteins recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. The TLRs act through adaptor molecules to activate various kinases and transcription factors so the organism can respond to potential infection. These adaptor molecules include MyD88, TIRAP, TIRP, TOLLIP, and TRIF. These molecules interact with and activate the IL-1R-associated kinase (IRAK) family, which then activates TNF receptor associated factor (TRAF)-6, and ultimately leads to the activation of NF-κB. While most TLRs utilize more than one adaptor, certain adaptor molecules are essential for individual TLR signaling, e.g., TLR4 signaling is dependent on TIRP expression.;;For images please see PDF data sheet

PD1 Detection Set
SD8600 1 Set
EUR 537.9
Description: PD-1 Antibody: Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antig en-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2. Upon binding to either of these ligands, signals generated by PD-1 inhibit the activation of the immune response in the absence of "danger signals" such as LPS or other molecules associated with bacteria or other pathogens. Evidence for this is seen in PD1-null mice who exhibit hyperactivated immune systems and autoimmune diseases.

IRAK Detection Set
PSI-1802 1 Set
EUR 884.4
Description: Members of the IRAK (IL-1R-associated kinase)/Pelle family play a major role in IL-1R/TLR mediated inflammatory responses and in innate immunity. IRAK and IRAK-2 regulate the activity of a signaling cascade that mediates the activation of NF-κB and MAP kinase. IRAK-4 interacts with and phosphorylates IRAK, while IRAK-M is thought to inhibit the recruitment and activation of IRAK-4 and IRAK. The importance of the IRAK family in inflammation and immunity is illustrated by the fact that animals lacking IRAK-4 are impaired in their responses to viral and bacterial challenges and are completely resistant to LPS challenge.;;For images please see PDF data sheet

ORAI Detection Set
PSI-1819 1 Set
EUR 1627.8
Description: Antigen stimulation of immune cells triggers Ca++ entry through Ca++ release-activated Ca++ (CRAC) channels. The ORAI family is a recently identified set of proteins that are essential components of these CRAC channels. A missense mutation in the ORAI1 protein in humans is the cause of one form of hereditary severe combined immune deficiency (SCID) which results in ablated T-cell Ca++ entry. It has been suggested that ORAI1 functions as a highly selective Ca++ plasma membrane channel that is gated through interactions with the stromal interaction molecule 1 (STIM1), the store-activated endoplasmic reticulum Ca++ sensor. Like ORAI1, ORAI2 also functions as a highly selective Ca++ plasma membrane channel that is gated through interactions with STIM1, although at a lesser efficacy than ORAI1. Although ORAI3 can also function as Ca++ plasma membrane channel, ORAI3 channels failed to produce detectable Ca++ selective currents in cells co-transfected with ORAI3 and STIM1, indicating that ORAI3 channels undergo a lesser degree of depotentiation than ORAI1 or ORAI2. Na+ currents through ORAI1, 2 and 3 channels were equally inhibited by extracellular Ca++, indicating that each have similar affinities for Ca++ within the selectivity filter. STIM1, in its function as a Ca++ sensor and an activator of CRAC channels, migrates to the plasma membrane from endoplasmic reticulum-like sites which act as cellular Ca++ stores. A related molecule, STIM2, inhibits the STIM1-mediated store-operated Ca++ entry, and can form complexes with STIM1, suggesting these two proteins may play a coordinated role in controlling Ca++ entry. The ORAI antibodies are predicted to have no cross-reactivity to the other ORAI proteins. Similarly, the STIM antibodies will not cross-react with the other STIM protein.;;For images please see PDF data sheet

Grik Detection Set
PSI-1824 1 Set
EUR 1627.8
Description: Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. Grik1, also known as glutamate receptor 5, belongs to the kainate family of glutamate receptors, which are composed of four subunits and function as ligand-activated ion channels. Grik1 is expressed in GABAergic interneurons of the hippocampus and are thought to participate in the formation of various subtypes of kainate receptors with Grik2 and Grik5/KA2. Stimulation of Grik1 leads to intracellular calcium release and activation of protein kinase C. Excessive activation has been associated with psychiatric, neurological and neurodegenerative diseases. Grik2, also known as glutamate receptor 6, may be associated with autosomal recessive mental retardation and possibly other neurological disorders such as schizophrenia. Numerous isoforms of Grik2 are known to exist and may be subject to RNA editing within the second transmembrane domain, which is thought to alter the properties of ion flow. Grik3, also known as glutamate receptor 7, has recently been shown to be an essential subunit of presynaptic kainate autoreceptors at hippocampal mossy fiber synapses as grik3-null mice show significantly reduced short- and long-term synaptic potentiation. Grik4 codes for the KA1 subunit of kainate-type ionotropic gluatamate receptors; mutations in this gene show significant association with both schizophrenia and bipolar disorder. Grik5, also known as kainate-preferring glutamate receptor subunit KA2, does not form homomeric channels, but instead forms heteromers with Grik2. In Grik2- but not Grik1-null mice, Grik5 surface expression is greatly reduced in neurons, indicating that Grik2/Grik5 heteromers are required for exit from the endoplasmic reticulum to the cell surface. ;;For images please see PDF data sheet

LAG3 Detection Set
SD8700 1 Set
EUR 569.4
Description: The lymphocyte activation gene-3 (LAG3) is a member of the immunoglobulin superfamily and binds MHC class II with high affinity (1), negatively regulating T-cell function and homeostasis (2). It is expressed in B, T, and NK cells, monocytes, and dendritic cells (3), and acts to regulate T cell expansion (4). LAG3 is also an important immune checkpoint protein, with anti-LAG3 antibodies activating T effector cells and affecting regulatory T cell functions. Furthermore LAG3 appears to act in a synergistic fashion with PD-1/PD-L1, suggesting that a dual antibody approach may prove useful in cancer immunotherapy.

PDL1 Detection Set
SD8500 1 Set
EUR 355.2
Description: Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antigen-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PD-L1 and PD-L2. PD-L1 is a B7-related protein that inhibits cell-mediated immune responses by reducing the secretion of IL-2 and IL-10 from memory T cells. This suggests that PD-L1 may be useful in reducing allogenic CD4+ memory T-cell responses to endothelial cells, thereby reducing the likelihood of host immune responses to allografts.

4x Detection Buffer
79389 10 ml
EUR 195
Description: The 4x Detection Buffer is optimized for use with the PRMT5 Homogeneous Assay Kit (BPS Bioscience #52052).

HMGB1 Detection Kit
6010 1 kit
EUR 903.9
Description: HMGB1 Detection Kit

OVA Detection ELISA
6032 1 kit
EUR 663.78
Description: OVA Detection ELISA

SAPAP Detection Set
PSI-1826 1 Set
EUR 752.1
Description: The members of the SAP90/PSD-95-associated protein (SAPAP) family (also known as the DLGAP family) specifically interact with PSD-95/SAP90, a membrane-associated guanylate kinase localized at postsynaptic density (PSD) in neuronal cells. The SAPAP proteins are thought to be adaptor proteins that also interact with different synaptic scaffolding proteins, cytoskeletal and signaling components, such as focal adhesion kinase (FAK) and proline-rich tyrosine kinase 2 (PYK2). SAPAP1, -2 and -4 mRNA are targeted to cell bodies, whereas SAPAP3 mRNA is detected mainly in cell bodies. SAPAP1 protein however, is targeted to the synapse and is not reliant on the synaptic localization of PSD-95 or the synaptic scaffolding molecule (S-SCAM). SAPAP3 protein is targeted to dendrites. Recent experiments have suggested that SAPAP3 may be involved in obsessive-compulsive disorder (OCD), as mice lacking SAPAP3 exhibited OCD-like symptoms which could be relieved by lentiviral-mediated selective expression of SAPAP3 in the striatum of SAPAP3-mutant mice. Multiple isoforms of the SAPAP proteins are known to exist.;;For images please see PDF data sheet

TIGIT Detection Set
SD8800 1 Set
EUR 569.4
Description: TIGIT Antibody: The T cell immunoreceptor with Ig and ITIM domains (TIGIT) is a member of the PVR (poliovirus receptor) family of immunoglobin proteins. It is expressed on several classes of T cells including follicular B helper T cells (TFH). TIGIT has been shown to bind PVR with high affinity; this binding is thought to assist interactions between TFH and dendritic cells to regulate T cell dependent B cell responses (1). Similar to other immune checkpoint proteins such as PD-1, TIGIT is upregulated on exhausted T cells in chronic viral infections and cancer. Blockade of both TIGIT and PD-1 pathways leads to tumor rejection in mice suggesting that it may be of therapeutic use against cancer (2).

TnI (Detection) Antibody
abx018057-100ug 100 ug
EUR 410.4

PCT (Detection) Antibody
20-abx019248
  • EUR 469.20
  • EUR 744.00
  • 0.5 mg
  • 1 mg

Apoptosis Detection Kit
ANXVKB-100T 100 test
EUR 557.64

Apoptosis Detection Kit
ANXVKCFB-100T 100 test
EUR 495.24

Apoptosis Detection Kit
ANXVKCFB7-100T 100 test
EUR 495.24

Apoptosis Detection Kit
ANXVKDY-100T 100 test
EUR 467.16

Apoptosis Detection Kit
ANXVKF-100T 100 test
EUR 395.4

Apoptosis Detection Kit
ANXVKF7-100T 100 test
EUR 395.4

Apoptosis Detection Kit
ANXVKPE-100T 100 test
EUR 415.68

CK19 (Detection) Antibody
abx019246-1mg 1 mg
EUR 794.4

Autophagy Detection Kit
abx299014-50tests 50 tests
EUR 627.6

Autophagy Detection Kit
abx299015-200tests 200 tests
EUR 1045.2

Matrillin Detection Set
PSI-1830 1 Set
EUR 752.1
Description: Matrilins (MATNs) are a family of non-collagenous extra-cellular matrix (ECM) proteins consisting of four known members that have been proposed to play key roles in modulating cellular phenotypes during chondrogenesis of mesenchymal stem cells (MSCs). MATN1 and MATN3 are expressed specifically in cartilage and are among the most up-regulated ECM proteins during chondrogenesis. MATN1 is composed of two Willebrand Factor A (vWFA) domains separated by one EGF-like domain, whereas MATN3 is composed of a single N-terminal vWFA domain followed by four epidermal growth factor (EGF) repeats and a coiled-coil domain. MATN1 or MATN3 may play a role in modulating chondrogenesis during the chondrocyte differentiation process. Mutations of MATN1 have been associated with variety of inherited chondrodysplasias, while aberrant expression and processing of MATN3 are hallmarks of conventional cartilaginous neoplasms. The MATN1 promoter region has also been shown to be associated with both susceptibility and disease progression in Adolescent idiopathic scoliosis. Other studies indicate that MATN2 is a permissive substrate for axonal growth and cell migration, and it is required for successful nerve regeneration, while MATN4 could serve as an odontoblast differentiation marker, e.g. in odontoblast stem cell research.;;For images please see PDF data sheet

Ubiquitin Detection Kit
SKT-131-20 20 assays
EUR 594
Description: Purification Detection kit used to capture, detect, identify and characterise ubiquitinated proteins and free chains from samples. in Cell Lysates, Tissue samples from all species

D-Dimer (Detection Ab)
abx019237-1mg 1 mg
EUR 644.4

Bradford(Coomassie) Protein Assay Plus Reagents
P7201-050 450ml
EUR 225.6

Senescence Detection Kit
55R-1370 250 tests
EUR 854.4
Description: Senescence Detection Kit for use in the research laboratory

Senescence Detection Kit
K2030-250 250 Staining
EUR 552

Senescence Detection Kit
K320-250 each
EUR 561.6

GenomeCoV19 Detection Kit
G628 100 Rxns
EUR 384
Description: The most affordable qPCR Detection Kit for SARS-CoV-2 on the market.abm’s GenomeCoV19 Detection Kit is a real-time reverse transcription-polymerase chain reaction (RT-qPCR) test intended for the qualitative detection of RNA from SARS-CoV-2 in human nasopharyngeal and oropharyngeal swab specimens from individuals suspected of COVID-19 by their healthcare provider. Our GenomeCoV19 Detection Kit is the most affordable qPCR detection kit on the market at only $1.77 USD/test (limited time pricing for US customers only).This kit is widely used in Europe under the CE-IVD certification and is listed by U.S. Food and Drug Administration (FDA) for distribution in the USA, under Section IV.C.

GenomeCoV19 Detection Kit
G628.v2 100 Rxns
EUR 384
Description: The most affordable qPCR Detection Kit for SARS-CoV-2 on the market.abm’s GenomeCoV19 Detection Kit is a real-time reverse transcription-polymerase chain reaction (RT-qPCR) test intended for the qualitative detection of RNA from SARS-CoV-2 in human nasopharyngeal and oropharyngeal swab specimens from individuals suspected of COVID-19 by their healthcare provider. Our GenomeCoV19 Detection Kit is the most affordable qPCR detection kit on the market at only $1.77 USD/test (limited time pricing for US customers only).This kit is widely used in Europe under the CE-IVD certification and is listed by U.S. Food and Drug Administration (FDA) for distribution in the USA, under Section IV.C.

Glutathione Detection Kit
SKT-202-96 1 plate of 96 wells
EUR 513.6
Description: Direct Fluorometric detection assay to measure the total GSH content in Whole Blood, Serum, EDTA Plasma, Heparin Plasma, Erythrocytes, Urine, Cell Lysates, Tissue samples from all species

SARS-CoV-2 IgG Detection Kit (Colorimetric Anti-Spike RBD IgG detection)
79985 96 rxns.
EUR 590
Description: The SARS-CoV-2 IgG detection kit is designed for qualitative detection of human IgG antibodies in serum collected from individuals suspected of prior infection with the virus that causes COVID-19. This fast and simple ELISA uses the receptor binding domain (RBD) of the SARS-CoV-2 Spike protein (BPS Bioscience #100696) to identify IgG antibodies that indicate a previous infection with SARS-CoV-2. 

Protein A Detection Kit
abx090813-1Kit 1 Kit
EUR 1897.2

NT-proBNP (Detection Ab)
abx019244-1mg 1 mg
EUR 828

PCR-STEC Detection Kit
K1452-96 each
EUR 940.8

Junctophilin Detection Set
PSI-1828 1 Set
EUR 752.1
Description: Junctional complexes between the plasma membrane (PM) and endoplasmic/sarcoplasmic reticulum (ER/SR) are a common feature of all excitable cell types and mediate cross talk between cell surface and intracellular ion channels. Junctophilins (JPs) are important components of the junctional complexes. JPs are composed of a carboxy-terminal hydrophobic segment spanning the ER/SR membrane and a remaining cytoplasmic domain that shows specific affinity for the PM. Four JPs have been identified as tissue-specific subtypes derived from different genes: JPH1 is expressed in skeletal muscle, JPH2 is detected throughout all muscle cell types, and JPH3 and JPH4 are predominantly expressed in the brain and contribute to the subsurface cistern formation in neurons. JPH1 is essential for stabilizing the T-tubule and SR membranes to form junctions and provide an environment for the assembly of receptors such as the ryanodine receptor type 1 (RyR1). JPH2-null mice died of embryonic cardiac arrest and human patients with mutations in the JPH2 gene showed hypertrophic cardiomyopathy, demonstrating the importance of this protein. Mice lacking both JPH3 and JPH4 subtypes exhibit serious symptoms such as impaired learning and memory and are accompanied by abnormal nervous functions. A repeat expansion in JPH3 is associated with Huntington disease-like 2. Multiple isoforms of the JPH proteins are known to exist.;;For images please see PDF data sheet

Rat Albumin Detection Kit
3020 1 kit
EUR 580.26
Description: Rat Albumin Detection Kit

TLR Adaptor Detection Set
PSI-1807 1 Set
EUR 884.4
Description: Toll-like receptors (TLRs) are evolutionarily conserved pattern-recognition molecules resembling the toll proteins that mediate antimicrobial responses in Drosophila. These proteins recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. The TLRs act through adaptor molecules to activate various kinases and transcription factors so the organism can respond to potential infection. These adaptor molecules include MyD88, TIRAP, TIRP, TOLLIP, and TRIF. These molecules interact with and activate the IL-1R-associated kinase (IRAK) family, which then activates TNF receptor associated factor (TRAF)-6, and ultimately leads to the activation of NF-κB. While most TLRs utilize more than one adaptor, certain adaptor molecules are essential for individual TLR signaling, e.g., TLR4 signaling is dependent on TIRP expression.;;For images please see PDF data sheet

SARS-CoV-2 IgG Detection Kit (Colorimetric Trimer Anti-Spike IgG detection)
79975 96 rxns.
EUR 765
Description: The SARS-CoV-2 IgG detection kit is designed for qualitative detection of human IgG antibodies in serum collected from individuals suspected of prior infection with the virus that causes COVID-19. This fast and simple ELISA uses the trimeric form of the SARS-CoV-2 Spike protein (BPS Bioscience #100728) to identify IgG antibodies that indicate a previous infection with SARS-CoV-2. The Spike protein is expressed on the viral membrane as a trimer, which means this kit measures IgG antibodies in a more physiologically relevant context than many other commercially available ELISA kits. 

Exosome ELISA Complete Kit (CD9 detection) 96 reactions
EXOEL-CD9A-1 96 reactions
EUR 956.4

Residual SDS Detection Kit
BSP055 100Assays
EUR 99.67

SARS Protein Detection Set
PSI-1810 1 Set
EUR 884.4
Description: A novel coronavirus has recently been identified as the causative agent of SARS (Severe Acute Respiratory Syndrome). Coronaviruses are a major cause of upper respiratory diseases in humans. The genomes of these viruses are positive-stranded RNA approximately 27-31kb in length. SARS infection can be mediated by the binding of the viral S (Spike) protein, a glycosylated 139 kDa protein and the major surface antigen of the virus, to the angiotensin-converting enzyme 2 (ACE2) on target cells. The M protein (Membrane protein, Matrix protein) is another major structural viral protein. It is an integral membrane protein involved in the budding of the viral particles and interacts with S protein and the nucleocapsid protein. The SARS E protein contains a short palindromic transmembrane helical hairpin that seems to deform lipid bilayers, which may explain its role in viral budding and virion envelope morphogenesis. ACE2, the SARS receptor, normally plays a central role in vascular, renal, and myocardial physiology. In contrast to its homolog ACE, ACE2 expression is restricted to heart, kidney, and testis.;;For images please see PDF data sheet

Nitric Oxide Detection Kit
SKT-212-96 2 plates of 96 wells
EUR 410.4
Description: Indirect Colorimetric assay used for quantitative measuring Nitrate and Nitrite present in a variety of samples in Serum, Plasma, Urine, Saliva, Water, Buffer, Cell Lysates, Tissue Culture Media samples from all species

4x DMT Detection Buffer 1
52301 10 ml
EUR 210
Description: The 4x Demethyltransferase Detection Buffer 1 is optimized for use with the following BPS Bioscience JMJD2 assay kits: ; • JMJD2A Homogeneous Assay Kit, #50413; • JMJD2B Homogeneous Assay Kit, #50414; • JMJD2C Homogeneous Assay Kit, #50415; • JMJD2E Homogeneous Assay Kit, #50417; • JMJD2C Chemiluminescent Assay Kit, #50405; • LSD1 Homogeneous Assay Kit, #50108.

PCR Mycoplasma Detection Kit
abx098883-100rxns 100 rxns
EUR 644.4

PCR Mycoplasma Detection Kit
abx298017-100rxns 100 rxns
EUR 376.8

Coliform Detection Broth 3mL
MIC6750 PK100
EUR 143.64

Listeria Detection Broth 3mL
MIC6756 PK100
EUR 180.12

A comparative research of acid, base and Fenton-like reagent handled biomass for Cr(VI) sequestration from aqueous options

All kinds of biomass supplies have been used for the removing of poisonous chromium(VI) by biosorption. The present research investigated the efficacy of Macadamia nutshells handled with sodium hydroxide, nitric acid, and the Fenton-like reagent within the removing of Cr(VI).

The adsorbents had been characterised by FTIR, SEM, TGA, and elemental evaluation. Results of useful parameters influencing the adsorption of Cr(VI), answer pH (pH 1-11), contact time (5-250 min), focus of adsorbent (1-10 g/L), and adsorbate focus (10-200 mg/L), had been investigated. The optimum situations for biosorption had been pH 1.4, adsorbent dose of 5 g/L, and 160 min of contact time.

In all circumstances, the base-treated adsorbent displayed superior efficiency in contrast to others, with highest % removing of 98%. The adsorbate-adsorbent interactions had been higher defined by the Freundlich isotherm and the pseudo-first-order charge mannequin. The Macadamia-based adsorbents is doubtlessly helpful for Cr(VI) removing from aqueous options.

Environment friendly Pd-Catalyzed Direct Coupling of Aryl Chlorides with Alkyllithium Reagents

Organolithium compounds are amongst a very powerful organometallic reagents and ceaselessly utilized in troublesome metallation reactions. Nevertheless, their direct use within the formation of C-C bonds is much less established.

Though outstanding advances within the coupling of aryl-lithium compounds have been achieved, Csp 2 -Csp three coupling reactions are very restricted. Right here, we report the primary basic protocol for the coupling or aryl chlorides with alkyllithium reagents.

  • Palladium catalysts primarily based on y lide-substituted phos phines (YPhos) had been discovered to be excellently suited to this transformation giving excessive selectivities at room temperature with quite a lot of aryl chlorides with out the necessity for an extra transmetallation reagent.
  • That is demonstrated in gram-scale synthesis together with constructing blocks for supplies chemistry and pharmaceutical business.
  • Moreover, the direct coupling of aryllithiums in addition to Grignard reagents with aryl chlorides was additionally simply completed at room temperature.

Atmospheric Stress Plasma Jet Therapy of Polymers Allows Reagent-Free Covalent Attachment of Biomolecules for Bioprinting

3D bioprinting, the place cells, hydrogels and structural polymers could be printed layer by layer into advanced designs, holds nice promise for advances in drugs and the biomedical sciences. In precept, this system allows the creation of extremely affected person particular illness fashions and biomedical implants. Nevertheless, a capability to tailor floor biocompatibility and interfacial bonding between printed parts, reminiscent of polymers and hydrogels, is presently missing. Right here we reveal that an atmospheric strain plasma jet (APPJ) can regionally activate polymeric surfaces for the reagent-free covalent attachment of proteins and hydrogel in a single-step course of at desired areas.
  • Polyethylene and poly--caprolactone (PCL) had been used as instance polymers. Covalent attachment of the proteins and hydrogel was demonstrated by resistance to removing by rigorous sodium dodecyl sulfate (SDS) washing.
  • The immobilized protein and hydrogel layers had been analyzed utilizing Fourier remodel infrared (FTIR) and X-ray photoelectron spectroscopy (XPS). Importantly, the APPJ floor activation additionally rendered the polymer surfaces mildly hydrophilic as required for optimum biocompatibility.
  • Water contact angles had been noticed to be steady inside a spread the place the conformation of biomolecules is preserved. Single and double electrode designs of APPJs had been in contrast of their traits related to localized floor functionalization, plume size and form.
  • As a proof of efficacy in a organic context, APPJ handled polyethylene functionalized with fibronectin was used to reveal enhancements in cell adhesion and proliferation.
  • These outcomes have essential implications for the event of a brand new technology of 3D bioprinters able to spatially patterned and tailor-made floor functionalization carried out through the 3D printing course of in situ.

ReadiLinkâ„¢ BSA Conjugation Kit
5501-2Reactions 2 Reactions
EUR 222
Description: Bovine serum albumin (BSA) is a serum albumin protein that has numerous biochemical applications including ELISAs (Enzyme-Linked Immunosorbent Assay), immunoblots, and immunohistochemistry.

ReadiLinkâ„¢ KLH Conjugation Kit
5502-2Reactions 2 Reactions
EUR 222
Description: Keyhole Limpet Hemocyanin (KLH) is one of the most commonly used carriers in the conjugation of peptides for antibody production.

CryoProtX Mix Reagents 1.5 mL
M-MDSR-61 1.5 ml ml
EUR 37
Description: CryoProtX Mix Reagents 1.5 mL

Antibody conjugation
CRO-6-Ind each Ask for price

Alkphos Direct Labelling Reagents
RPN3680 EACH
EUR 813.6

ReadiLink™ BSA Conjugation Kit
5501 1 kit
EUR 222

ReadiLink™ KLH Conjugation Kit
5502 1 kit
EUR 222

Set of 10 Biolipidure Reagents
Biolipidure-set 10mLx10
EUR 1820.4
Description: Set of 10 Biolipidure Reagents, whose applications include Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement

Buccutiteâ„¢ Poly-HRP Antibody Conjugation Kit
5518-1x50ugLabeling 1x50 ug Labeling
EUR 412
Description: Poly-HRP-antibody conjugates is considered to have the highest sensitivity in immunoassays where the sample is limited, or a target molecule is present at extremely low level.

Buccutiteâ„¢ Poly-HRP Antibody Conjugation Kit
5519-2x50ugLabelings 2x50 ug Labelings
EUR 516
Description: Poly-HRP-antibody conjugates is considered to have the highest sensitivity in immunoassays where the sample is limited, or a target molecule is present at extremely low level.

CryoProtX Mix Eco Reagents 1.5 mL
M-MDSR-61-ECO 1.5 ml ml
EUR 37
Description: CryoProtX Mix Eco Reagents 1.5 mL

Free Chlorine Reagents 0-5mg/L
WAT1028 EACH
EUR 44.4

Buccutite™ Poly-HRP Antibody Conjugation Kit
5518 1x50 ug Labeling
EUR 412

Buccutite™ Poly-HRP Antibody Conjugation Kit
5519 2x50 ug Labelings
EUR 516

ReadiLinkâ„¢ Protein Conjugation Stop Buffer
5400-1ml 1 ml
EUR 86
Description: ReadiLink™ Protein Conjugation Stop Buffer is used to stop the protein conjugation reactions that use succinimidyl ester, sulfonyl chloride or isothiocyanate.

ReadiLink™ Protein Conjugation Stop Buffer
5400 1 ml
EUR 86

Bradford(Coomassie) Protein Assay Plus Reagents
P7201-050 450ml
EUR 225.6

Horseradish peroxidase (HRP) conjugation kit for antigen/antibodies (Micro; 3 x 200 ug HRP)
80225 1 kit
EUR 534

Horseradish peroxidase (HRP) conjugation kit for antigen/antibodies (1x 2 mg)
80220 1 Kit
EUR 534

FITC Antibody Conjugation Kit (OKCC00001)
OKCC00001 1 Unit
EUR 255.6
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

Biotin Antibody Conjugation Kit (OKCC00007)
OKCC00007 1 Unit
EUR 255.6
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

ASB-415 Antibody Conjugation Kit (OKCC00002)
OKCC00002 1 Unit
EUR 255.6
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

ASB-490 Antibody Conjugation Kit (OKCC00003)
OKCC00003 1 Unit
EUR 255.6
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

ASB-550 Antibody Conjugation Kit (OKCC00004)
OKCC00004 1 Unit
EUR 255.6
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

ASB-650 Antibody Conjugation Kit (OKCC00005)
OKCC00005 1 Unit
EUR 255.6
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

ASB-750 Antibody Conjugation Kit (OKCC00006)
OKCC00006 1 Unit
EUR 255.6
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

Horseradish Peroxidase *Optimized for conjugation*
11021 10 mg
EUR 82

Horseradish Peroxidase *Optimized for conjugation*
11021-10mg 10 mg
EUR 82
Description: Horseradish peroxidase (HRP) is a glycoprotein with a molecular weight of ~44 KD.

Buccutiteâ„¢ Peroxidase (HRP) Antibody Conjugation Kit *Optimized for Labeling 100 ug Protein*
5503-2Labelings 2 Labelings
EUR 265
Description: Protein-protein conjugations are commonly performed with a bifunctional linker (such as the commonly used SMCC), having different reactivity on each end for linking two different proteins.

Buccutiteâ„¢ Peroxidase (HRP) Antibody Conjugation Kit *Optimized for Labeling 1 mg Protein*
5504-1Labeling 1 Labeling
EUR 532
Description: Protein-protein conjugations are commonly performed with a bifunctional linker (such as the commonly used SMCC), having different reactivity on each end for linking two different proteins.

Buccutiteâ„¢ Peroxidase (HRP) Antibody Conjugation Kit *Optimized for Labeling 25 ug Protein*
5505-2Labelings 2 Labelings
EUR 211
Description: Protein-protein conjugations are commonly performed with a bifunctional linker (such as the commonly used SMCC), having different reactivity on each end for linking two different proteins.

Buccutiteâ„¢ Peroxidase (HRP) Antibody Conjugation Kit *Optimized for Labeling 1 mg Protein*
5506-5Labelings 5 Labelings
EUR 1555
Description: Protein-protein conjugations are commonly performed with a bifunctional linker (such as the commonly used SMCC), having different reactivity on each end for linking two different proteins.

Maleimide Activated KLH-Peptide Conjugation Kit
K2039-5 5 columns
EUR 538.8

Buccutite™ Peroxidase (HRP) Antibody Conjugation Kit *Optimized for Labeling 100 ug Protein*
5503 1 kit
EUR 265

Buccutite™ Peroxidase (HRP) Antibody Conjugation Kit *Optimized for Labeling 1 mg Protein*
5504 1 kit
EUR 532

Buccutite™ Peroxidase (HRP) Antibody Conjugation Kit *Optimized for Labeling 25 ug Protein*
5505 2 Labelings
EUR 211

Buccutite™ Peroxidase (HRP) Antibody Conjugation Kit *Optimized for Labeling 1 mg Protein*
5506 5 Labelings
EUR 1555

Gold-in-a-Box Conjugation Kit, 20 nm, 18 mL
NGIB20-B018 18 mL
EUR 784.18
Description: Gold-in-a-Box Conjugation Kitis used to prepare highly-reactive antibody or soluble protein gold conjugates 20 nm that can be utilized in lateral-flow assays.

Gold-in-a-Box Conjugation Kit, 20 nm, 44 mL
NGIB20-B044 44 mL
EUR 1685.11
Description: Gold-in-a-Box Conjugation Kitis used to prepare highly-reactive antibody or soluble protein gold conjugates 20 nm that can be utilized in lateral-flow assays.

Gold-in-a-Box Conjugation Kit, 20 nm, 100 mL
NGIB20-B100 100 mL
EUR 3141.24
Description: Gold-in-a-Box Conjugation Kitis used to prepare highly-reactive antibody or soluble protein gold conjugates 20 nm that can be utilized in lateral-flow assays.

Gold-in-a-Box Conjugation Kit, 40 nm, 18 mL
NGIB40-B018 18 mL
EUR 784.18
Description: Gold-in-a-Box Conjugation Kitis used to prepare highly-reactive antibody or soluble protein gold conjugates 40 nm that can be utilized in lateral-flow assays.

Gold-in-a-Box Conjugation Kit, 40 nm, 44 mL
NGIB40-B044 44 mL
EUR 1685.11
Description: Gold-in-a-Box Conjugation Kitis used to prepare highly-reactive antibody or soluble protein gold conjugates 40 nm that can be utilized in lateral-flow assays.

Gold-in-a-Box Conjugation Kit, 40 nm, 100 mL
NGIB40-B100 100 mL
EUR 3141.24
Description: Gold-in-a-Box Conjugation Kitis used to prepare highly-reactive antibody or soluble protein gold conjugates 40 nm that can be utilized in lateral-flow assays.

Ubiquitin Conjugation Factor E4 A (UBE4A) Antibody
abx239192-100ug 100 ug
EUR 610.8

Ubiquitin Conjugation Factor E4 B (UBE4B) Antibody
abx239193-100ug 100 ug
EUR 610.8

Ubiquitin Conjugation Factor E4 B (UBE4B) Antibody
20-abx325296
  • EUR 376.80
  • EUR 292.80
  • 100 ug
  • 50 ug

Ubiquitin Conjugation Factor E4 A (UBE4A) Antibody
20-abx322075
  • EUR 526.80
  • EUR 393.60
  • 100 ul
  • 50 ul

Ubiquitin Conjugation Factor E4 A (UBE4A) Antibody
20-abx327343
  • EUR 376.80
  • EUR 292.80
  • 100 ug
  • 50 ug

Ubiquitin Conjugation Factor E4 A (UBE4A) Antibody
abx031521-400ul 400 ul
EUR 627.6

Ubiquitin Conjugation Factor E4 A (UBE4A) Antibody
abx031521-80l 80 µl
EUR 343.2

Ubiquitin Conjugation Factor E4 A (UBE4A) Antibody
abx122732-100ug 100 ug
EUR 469.2

Ubiquitin Conjugation Factor E4 A (UBE4A) Antibody
20-abx002394
  • EUR 493.20
  • EUR 710.40
  • EUR 218.40
  • EUR 376.80
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

Ubiquitin Conjugation Factor E4 A (UBE4A) Antibody
20-abx008428
  • EUR 360.00
  • EUR 526.80
  • EUR 226.80
  • 100 ul
  • 200 ul
  • 30 ul

Antibody/Protein conjugation to FITC kit (sufficient for 2 reactions)
80230 1 kit
EUR 534

Amersham ECL Western Blotting Detection Reagents for 4000cm2 membrane
RPN2106 EACH
EUR 374.4

Amersham ECL Western Blotting Detection Reagents for 1000cm2 membrane
RPN2109 EACH
EUR 188.4

Amersham ECL Western Blotting Detection Reagents for 6000cm2 membrane
RPN2134 1KIT
EUR 572.4

Amersham ECL Western Blotting Detection Reagents for 2000cm2 membrane
RPN2209 EACH
EUR 291.6

DiagNano Gold Nanoparticle Passive Conjugation Kit, TEM
GPK-TEM 1 kit
EUR 976.8

Gold-in-a-Box Conjugation Kit, 20 nm & 40 nm, 18 mL
NGIB24-B018 18 mL
EUR 784.18
Description: Gold-in-a-Box Conjugation Kitis used to prepare highly-reactive antibody or soluble protein gold conjugates 20 or 40 nm that can be utilized in lateral-flow assays.

Gold-in-a-Box Conjugation Kit, 30 nm & 40 nm, 18 mL
NGIB34-B018 18 mL
EUR 784.18
Description: Gold-in-a-Box Conjugation Kitis used to prepare highly-reactive antibody or soluble protein gold conjugates 30 or 40 nm that can be utilized in lateral-flow assays.

DiagNano Gold Nanoparticle Passive Conjugation Kit, 100 nm
GPK-100 1 kit
EUR 858

DiagNano Gold Nanoparticle Passive Conjugation Kit, 15 nm
GPK-15 1 kit
EUR 858

DiagNano Gold Nanoparticle Passive Conjugation Kit, 20 nm
GPK-20 1 kit
EUR 858

DiagNano Gold Nanoparticle Passive Conjugation Kit, 30 nm
GPK-30 1 kit
EUR 858

DiagNano Gold Nanoparticle Passive Conjugation Kit, 40 nm
GPK-40 1 kit
EUR 858

DiagNano Gold Nanoparticle Passive Conjugation Kit, 5 nm
GPK-5 1 kit
EUR 858

DiagNano Gold Nanoparticle Passive Conjugation Kit, 50 nm
GPK-50 1 kit
EUR 858

DiagNano Gold Nanoparticle Passive Conjugation Kit, 60 nm
GPK-60 1 kit
EUR 858

DiagNano Gold Nanoparticle Passive Conjugation Kit, 70 nm
GPK-70 1 kit
EUR 858

DiagNano Gold Nanoparticle Passive Conjugation Kit, 80 nm
GPK-80 1 kit
EUR 858

Human Ubiquitin Conjugation Factor E4 A (UBE4A) ELISA Kit
abx384086-96tests 96 tests
EUR 1093.2

Human Ubiquitin Conjugation Factor E4 B (UBE4B) ELISA Kit
abx384087-96tests 96 tests
EUR 1093.2

Mouse Ubiquitin conjugation factor E4 B, Ube4b ELISA KIT
ELI-40412m 96 Tests
EUR 1038

Human Ubiquitin conjugation factor E4 B, UBE4B ELISA KIT
ELI-28628h 96 Tests
EUR 988.8

Latex-in-a-Box Conjugation Kit, Red 0.3 micron, 7 mL
LIB03-R007 7 mL
EUR 880.73
Description: Latex-in-a-Box Conjugation Kit conjugates red 0.3 micron latex nanoparticles to antibodies and proteins. The nanoparticles are created using a special technology that needs only one hour to conjugate the particles to the antibody/protein.

Latex-in-a-Box Conjugation Kit, Blue 0.3 micron, 7 mL
LIB03-B007 7 mL
EUR 880.73
Description: Latex-in-a-Box Conjugation Kit conjugates blue 0.3 micron latex nanoparticles to antibodies and proteins. The nanoparticles are created using a special technology that needs only one hour to conjugate the particles to the antibody/protein.

Latex-in-a-Box Conjugation Kit, Green 0.2 micron, 7 mL
LIB02-G007 7 mL
EUR 880.73
Description: Latex-in-a-Box Conjugation Kit conjugates green 0.2 micron latex nanoparticles to antibodies and proteins. The nanoparticles are created using a special technology that needs only one hour to conjugate the particles to the antibody/protein.

Latex-in-a-Box Conjugation Kit, Orange 0.2 micron, 7 mL
LIB02-O007 7 mL
EUR 880.73
Description: Latex-in-a-Box Conjugation Kit conjugates orange 0,2 micron latex nanoparticles to antibodies and proteins. The nanoparticles are created using a special technology that needs only one hour to conjugate the particles to the antibody/protein.

DiagNano Gold Nanoparticle Passive Conjugation Kit, Dot-blot
GPK-DB 1 kit
EUR 976.8

Peptide/Protein conjugation to Pre-activated BSA Kit (sufficient for 4 reactions)
80240 1 kit
EUR 489.6

Peptide/Protein conjugation to Pre-activated KLH Kit (sufficient for 4 reactions)
80250 1 kit
EUR 489.6

Absolut Mag Carboxyl Magnetic Particles Conjugation Kit, 50 nm
WHM-K024 10 mg
EUR 1232.4

Absolut Mag Carboxyl Magnetic Particles Conjugation Kit, 100 nm
WHM-K026 10 mg
EUR 1232.4

Absolut Mag Carboxyl Magnetic Particles Conjugation Kit, 150 nm
WHM-K027 10 mg
EUR 1232.4

Absolut Mag Carboxyl Magnetic Particles Conjugation Kit, 200 nm
WHM-K028 10 mg
EUR 1232.4

Absolut Mag Carboxyl Magnetic Particles Conjugation Kit, 500 nm
WHM-K029 20 mg
EUR 1076.4

Absolut Mag Carboxyl Magnetic Particles Conjugation Kit, 1 µm
WHM-K030 20 mg
EUR 1076.4

Rat UBE4A (Ubiquitin conjugation factor E4 A) ELISA Kit (CUSTOM)
ELI-40411r 96 Tests
EUR 1063.2
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