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August 26, 2020

Western Blotting by Direct Deposition of Reagents onto Capture Membrane

By Danielle

Fast Immunoassay for Microfluidic Western Blotting by Direct Deposition of Reagents onto Capture Membrane

Western blotting is a widely used protein assay platform, but the technique requires long analysis times and multiple manual steps. Microfluidic systems are currently being explored for increased automation and reduction of analysis times, sample volumes, and reagent consumption for western blots. Previous work has demonstrated that proteins separated by microchip electrophoresis can be captured on membranes by dragging the microchip outlet across the membrane.
This process reduces the separation and transfer time of a western blot to a few minutes. To further improve the speed and miniaturization of a complete western blot, a microscale immunoassay with direct deposition of immunoassay reagents has been developed. Flow deposition of antibodies is used to overcome diffusion limited binding kinetics so that the entire immunoassay can be completed in 1 h with detection sensitivity comparable to incubation steps requiring 20 h. The use of low microliter/min flow rates with antibody reagents applied directly and locally to the membrane where the target proteins have been captured, reduced antibody consumption ~30-fold. The complete western blot was applied to the detection of GAPDH and β-Tubulin from A431 cell lysate.

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Agarose 3:1 High Resolution Blend
40100080-2 100 g
EUR 190.48

Agarose 3:1 High Resolution Blend
40100080-3 250 g
EUR 381.93

Agarose MR, Medium Resolution
40100132-1 100 g
EUR 284.99

Agarose MR, Medium Resolution
40100132-2 500 g
EUR 917.75

Apex Super Resolution Agarose
20-106 100g/Unit
EUR 275.39
Description: 3:1 Blend, Ultra Pure

Agarose SFR, Super Fine Resolution
40100140-1 25 g
EUR 81.92

Agarose SFR, Super Fine Resolution
40100140-2 100 g
EUR 204.8

Agarose SFR, Super Fine Resolution
40100140-3 250 g
EUR 506.88

Apex Super Fine Resolution Agarose
20-239 100g/Unit
EUR 303.31
Description: Ultra Pure

Apex Super Fine Resolution Agarose
20-240 250g/Unit
EUR 781.72
Description: Ultra Pure

Agarose: high melting temperature/medium resolution for all of your standard DNA applications 500 g - EACH
DD4905009 EACH
EUR 1391.85

5.7x14 cellogel 190µ for high resolution (25)
EHCA1200-ST33-25 ea
EUR 63.6

high resolution serium porteins kit incipien g ...
EHCA1200-KC31 ea
EUR 192

High resolution protein SDS-PAGE electrophoresis pre mixed gel Kit (BIS Tris system)
E1WP3231 50T
EUR 100

High resolution protein SDS-PAGE electrophoresis pre mixed gel Kit (BIS Tris system)
E1WP3232 100T
EUR 150

Resolution Std MeOH Blank - 100ML
RSPEC-EPR003 100ML
EUR 304.04

Resolution Std Toluene in MeOH - 100ML
RSPEC-EPR002 100ML
EUR 304.04

QRRPT1 Rapid Run™ (High Sub) Agarose Bead
QRRPT1-25 25 ml
EUR 72.04

QRRPT1 Rapid Run™ (High Sub) Agarose Bead
QRRPT1-300 300 ml
EUR 497.73

BioDrop Resolution CFR PC software - EACH
SPE6056 EACH
EUR 1962.9

EP Reagent Buffered Acetone Sol. - 1L
4000100 1L
EUR 82.35

Agarose, Resolute GPG
A2503-100000 100 g
EUR 477.6
Description: resolve differences in bp length by as little as 2%finely resolve DNA fragments from 20-1000bp, rivals polyacrylamidegels are extremely clear and easy to handle due to high gel strengthno detectable inhibition of DNA ligase or the following restriction enzymes:BamH I, Hind II, EcoR I..

10X PHOSPHATE BUFFERED SALINE Reagent
GWB-Q00202 1 LITRE Ask for price

TRIS BUFFERED SALINE WITH TWEEN 20 Reagent
GWB-Q00297 500 ml Ask for price

Hanna Pocket Tester pHECTDSoC Hi Resolution - EACH
PHM4132 EACH
EUR 306.45

Agarose Nr.4 for EID (Laurell)
MBS570408-10g 10g
EUR 275

Agarose Nr.4 for EID (Laurell)
MBS570408-5x10g 5x10g
EUR 1090

Resolution Std Toluene in MeOH w/ Blank in Cuvettes - PK2
RSPEC-EPR001 PK2
EUR 1650.93

Hanna Checker pH Tester HI 98103 with 0.1 pH Resolution - EACH
PHM4000 EACH
EUR 87.75

REAGENTS HIGH RANGE AMMONIA - PK25
HI73325 PK25
EUR 55.35

High Range Ammonia Reagents - EACH
HI9373301 EACH
EUR 130.95

RFM340-T digital refractometer 0.000 Bx Resolution Touch Display - EACH
REF1148 EACH
EUR 12995.94

RFM330-T digital refractometer 0.0 Bx Resolution Touch Display - EACH
REF1144 EACH
EUR 12130.82

Biotin Agarose Resin
4BCL-BI-10 10 ml
EUR 466.21

Biotin Agarose Resin
4BCL-BI-5 5 ml
EUR 245.37

Protein A Agarose IP Reagent
abx097210-2ml 2 ml
EUR 326.4

Protein G Agarose IP Reagent
abx097211-2ml 2 ml
EUR 326.4

Protein A Agarose IP Reagent
abx097210-100l 100 µl
EUR 200

Protein A Agarose IP Reagent
abx097210-1ml 1 ml Ask for price

Protein A Agarose IP Reagent
abx097210-200l 200 µl Ask for price

Protein G Agarose IP Reagent
abx097211-100l 100 µl
EUR 200

Protein G Agarose IP Reagent
abx097211-1ml 1 ml Ask for price

Protein G Agarose IP Reagent
abx097211-200l 200 µl Ask for price

Protein G Agarose IP Reagent
MBS826724-2mL 2mL
EUR 185

Protein G Agarose IP Reagent
MBS826724-5x2mL 5x2mL
EUR 750

Protein A Agarose IP Reagent
MBS826731-2mL 2mL
EUR 185

Protein A Agarose IP Reagent
MBS826731-5x2mL 5x2mL
EUR 750

Glutathione Agarose Resin
4B-GLU-10 10 ml
EUR 178.66

Glutathione Agarose Resin
4B-GLU-100 100 ml
EUR 1284.3

Protein L Agarose Resin
4BCLPL-10 10 ml
EUR 417.67

Protein L Agarose Resin
4BCLPL-2 2 ml
EUR 185.4

Protein L Agarose Resin
4BCLPL-5 5 ml
EUR 289.22

Nickel NTA Agarose Resin
6BCL-NTANi-100 100 ml
EUR 836.17

Nickel NTA Agarose Resin
6BCL-NTANi-25 25 ml
EUR 242.1

Nickel NTA Agarose Resin
6BCL-NTANi-500 500 ml
EUR 3483.72

Protein A Agarose Resin
PA09-100 100 ml
EUR 2300

Protein A Agarose Resin
PA09-25 25 ml
EUR 1047.15

Protein A Agarose Resin
PA09-5 5 ml
EUR 297.05

Protein A Agarose Resin
PA09-500 500 ml
EUR 4070

Protein A&G Agarose IP Reagent
abx097212-2ml 2 ml
EUR 326.4

Protein A&G Agarose IP Reagent
abx097212-100l 100 µl
EUR 200

Protein A&G Agarose IP Reagent
abx097212-1ml 1 ml Ask for price

Protein A&G Agarose IP Reagent
abx097212-200l 200 µl Ask for price

Protein A&G Agarose IP Reagent
MBS826733-2mL 2mL
EUR 185

Protein A&G Agarose IP Reagent
MBS826733-5x2mL 5x2mL
EUR 750

Pro Live-Cell Tubulin Staining Kit (Green Fluorescence with Super Resolution)
MBS9718801-1000Tests 1000Tests
EUR 1920

Pro Live-Cell Tubulin Staining Kit (Green Fluorescence with Super Resolution)
MBS9718801-250Tests 250Tests
EUR 625

Pro Live-Cell Tubulin Staining Kit (Green Fluorescence with Super Resolution)
MBS9718801-50Tests 50Tests
EUR 280

Pro Live-Cell Tubulin Staining Kit (Green Fluorescence with Super Resolution)
MBS9718801-5x1000Tests 5x1000Tests
EUR 8920

Streptavidin 6HC Agarose Resin
STV6HC-10 10 ml
EUR 472.71

Streptavidin 6HC Agarose Resin
STV6HC-20 20 ml
EUR 850.88

Streptavidin 6HC Agarose Resin
STV6HC-5 5 ml
EUR 259.99

Protein A Mag1 Agarose Resin
PA-MAG1-2 2ml beads
EUR 90

Protein A Mag1 Agarose Resin
PA-MAG1-5 5ml beads
EUR 215

Protein G Mag1 Agarose Resin
PG-MAG1-2 2ml beads
EUR 96

Protein G Mag1 Agarose Resin
PG-MAG1-5 5ml beads
EUR 225

Protein L Mag1 Agarose Resin
PL-MAG1-2 2ml beads
EUR 105

Protein L Mag1 Agarose Resin
PL-MAG1-5 5ml beads
EUR 247

Agarose (3%) & LE Agarose 1% in 1X TAE Buffer
40120844-1 100 mL
EUR 33.86

Agarose (3%) & LE Agarose 1% in 1X TAE Buffer
40120844-2 250 mL
EUR 53.99

Agarose (3%) & LE Agarose 1% in 1X TAE Buffer
40120844-3 500 mL
EUR 81.44

Protein A Agarose Resin 4 Rapid Run™
4RRPA-25 25 ml
EUR 1514.98

Protein A Agarose Resin 4 Rapid Run™
4RRPA-5 5 ml
EUR 409.12

Protein A Agarose Resin 4 Rapid Run™
4RRPA-500 500 ml
EUR 4270

Protein G Agarose Resin 4 Rapid Run™
4RRPG-100 100 ml
EUR 1699.5

Protein G Agarose Resin 4 Rapid Run™
4RRPG-25 25 ml
EUR 1002.55

Establishment of a candidate equine influenza Florida Clade 2 strain A/eq/Richmond/1/07 horse antiserum as Ph. Eur. Biological Reference Preparation/OIE International Reference Reagent

Equine influenza (EI) is an important respiratory disease of horses, with welfare and economic consequences. Vaccination remains one of the most efficient prevention methods available. Equine influenza virus (EIV) is constantly evolving and consequently EI vaccines need to be updated on a regular basis. In 2010, the World Organisation for Animal Health (OIE) Expert Surveillance Panel (ESP) on EI provided a new recommendation for EI vaccine strain composition, including the incorporation of representative EIV strains of both Florida Clade 1 and Clade 2 sub-lineages (FC1 and FC2, respectively). In this context, the European Pharmacopoeia (Ph. Eur.) – OIE reference panel for EI had to be complemented by an antiserum raised in horses against the FC2 representative EIV strain A/eq/Richmond/1/07.
An international collaborative study was organised and managed by the European Directorate for the Quality of Medicines and HealthCare (EDQM) within the framework of its Biological Standardisation Programme (BSP). The study aimed at evaluating a new candidate reference for use as a common OIE International Standard/Ph. Eur. Biological Reference Preparation (BRP) horse antiserum to FC2 EIV A/equine/Richmond/1/07.
The standard was to be established using the SRH and HI tests for subsequent use in immunogenicity, efficacy and batch potency assay of EI vaccines as a Ph. Eur. BRP (Ph. Eur. monograph 0249) and for use in clinical diagnostic tests as an OIE-approved International Standard Reagent (OIE chapter 3.5.7). The collaborative study confirmed the suitability of the candidate and an SRH titre was assigned. The candidate was adopted as a BRP by the Ph. Eur. Commission and approved by the OIE Biological Standards Commission as an International Standard Serum in November 2017 and February 2018, respectively.

Selective phase transfer reagents (OxP-crowns) for chromogenic detection of nitrates especially ammonium nitrate

Nitrogen and phosphorus-containing ions such as ammonium, nitrates and phosphates are anthropogenic pollutants while ammonium nitrate may be diverted for nefarious purposes in improvised explosive devices. Crown ether-oxoporphyrinogen conjugates (OxP-crowns) are used to selectively detect nitrates, especially their ion pairs with K+ and NH4+, based on ion pair complexation of OxP-crowns under phase transfer conditions.
The presence of phosphate and carbonate lead to deprotonation of OxP-crowns. OxP-1N18C6 is capable of extracting ion pairs with nitrate from aqueous phase leading to a selective chromogenic response. Deprotonation of the OxP moiety leads to [OxP-]-1N18C6[K+] and is promoted by crown ether selective cation binding coupled with hydration of basic oxoanions, which are constrained to remain in the aqueous phase.
This work illustrates the utility of molecular design to exploit partitioning and ion hydration effects establishing selectivity of chromogenic response.

CD45RA / CD45RO / CD3 / CD8 Antibody (FITC, PE, PerCP, APC)
abx200641-50tests 50 tests
EUR 828

CD45RA / CD62L / CD3 / CD8 Antibody (FITC, PE, PerCP, APC)
abx200643-50tests 50 tests
EUR 828

CD45RA FITC
MBS396553-100Tests 100Tests
EUR 310

CD45RA FITC
MBS396553-5x100Tests 5x100Tests
EUR 1250

CD4 / CD45RA Antibody (FITC, PE)
abx200601-50tests 50 tests
EUR 678

CD45RA / CD4 Antibody (FITC, PE)
abx200611-50tests 50 tests
EUR 526.8

CD45RA, B-C15; IgG2a; FITC; 100tests/1ml
M854321010 1 unit
EUR 273.63

COMBI IC Reagent: Mouse anti CD45 (FITC) and Mouse anti CD14 (PE)
MBS570370-50Tests 50Tests
EUR 450

COMBI IC Reagent: Mouse anti CD45 (FITC) and Mouse anti CD14 (PE)
MBS570370-5x50Tests 5x50Tests
EUR 1885

EZ-DNA Reagents
BS8202 100preps
EUR 105.67

EZ-DNA Reagents
SK8201 100preps
EUR 112.2

CD45RA Antibody (FITC)
abx139834-100tests 100 tests
EUR 510

CD45RA Antibody (FITC)
abx134000-100Assays 100 Assays
EUR 526.8

CD45RA Antibody (FITC)
abx134000-50Assays 50 Assays
EUR 410.4

CD45RA Antibody (FITC)
abx200319-100tests 100 tests
EUR 510

CD45RA Antibody (FITC)
abx200323-100tests 100 tests
EUR 477.6

CD45RA Antibody (FITC)
GWB-5A46D4 0.1 mg Ask for price

CD45RA Antibody (FITC)
GWB-85C3B6 0.02 mg Ask for price

CD45RA Antibody (FITC)
GWB-91139F 0.1 mg Ask for price

CD45RA Antibody (FITC)
GWB-A6BC25 0.1 mg Ask for price

CD45RA Antibody (FITC)
GWB-F8A7CB 100 TESTS Ask for price

CD45RA Antibody (FITC)
GWB-D84ABE 0.025 mg Ask for price

CD45RA Antigen (CD45RA/PTPRC) Antibody (FITC)
abx413328-01mg 0.1 mg
EUR 777.6

CD45RA Antigen (CD45RA/PTPRC) Antibody (FITC)
abx414287-01mg 0.1 mg
EUR 610.8

CD45RA Antigen (CD45RA/PTPRC) Antibody (FITC)
abx414687-01mg 0.1 mg
EUR 610.8

CD45RA Antigen (CD45RA/PTPRC) Antibody (FITC)
abx415608-01mg 0.1 mg
EUR 610.8

CD45RA Antigen (CD45RA/PTPRC) Antibody (FITC)
abx415609-25ug 25 ug
EUR 326.4

CD45RA Antigen (CD45RA/PTPRC) Antibody (FITC)
abx139834-100g 100 µg
EUR 350

CD45RA Antigen (CD45RA/PTPRC) Antibody (FITC)
abx228507-100g 100 µg
EUR 62.5

CD45RA Antigen / PTPRC (CD45RA) Antibody (FITC)
abx413328-200l 200 µl
EUR 562.5

CD45RA Antigen / PTPRC (CD45RA) Antibody (FITC)
abx414687-200l 200 µl
EUR 425

CD45RA Antigen / PTPRC (CD45RA) Antibody (FITC)
abx415609-200l 200 µl
EUR 175

CD8 (FITC) CD38 (RPE) Antibody
GWB-Q01600 50 TESTS Ask for price

Ecl Detection Reagents
RPN3004 EACH
EUR 552.9

CD3 (FITC) CD8 (RPE) Antibody
GWB-Q01593 50 TESTS Ask for price

CD8 (FITC) CD4 (RPE) Antibody
GWB-Q01601 50 TESTS Ask for price

Anti-Hu CD45RA FITC
1F-223-T025 25 tests
EUR 99

Anti-Hu CD45RA FITC
1F-223-T100 100 tests
EUR 198

Anti-Hu CD45RA FITC
MBS1801237-100Tests 100Tests
EUR 305

Anti-Hu CD45RA FITC
MBS1801237-5x100Tests 5x100Tests
EUR 1120

CD45RA Mouse mAb (FITC)
MBS4753563-01mL 0.1mL
EUR 450

CD45RA Mouse mAb (FITC)
MBS4753563-5x01mL 5x0.1mL
EUR 1540

FITC anti-rat CD45RA
E16FRF045RA-100U 100 μg
EUR 563.33
Description: Available in various conjugation types.

Mouse Human CD45RA (FITC)
E2790281 100ul
EUR 225
Description: Available in various conjugation types.

Mouse Human CD45RA (FITC)
MBS8559305-01mL 0.1mL
EUR 305

Mouse Human CD45RA (FITC)
MBS8559305-01mLAF405L 0.1mL(AF405L)
EUR 565

Mouse Human CD45RA (FITC)
MBS8559305-01mLAF405S 0.1mL(AF405S)
EUR 565

Mouse Human CD45RA (FITC)
MBS8559305-01mLAF610 0.1mL(AF610)
EUR 565

Mouse Human CD45RA (FITC)
MBS8559305-01mLAF635 0.1mL(AF635)
EUR 565

Ecl Detection Reagents - EACH
RPN2105 EACH
EUR 815.4

FITC anti-human CD45RA
E16FHF0453-025 25 tests
EUR 106.67
Description: Available in various conjugation types.

FITC anti-human CD45RA
E16FHF0453-050 50 tests
EUR 152.33
Description: Available in various conjugation types.

FITC anti-human CD45RA
E16FHF0453-100 100 Tests
EUR 228.67
Description: Available in various conjugation types.

Rat CD45RA Antibody (FITC)
GWB-1A46AD 0.1 mg Ask for price

Pig CD45RA Antibody (FITC)
GWB-Q01216 0.1 mg Ask for price

Anti-Human CD45RA FITC
MBS696724-100Tests 100Tests
EUR 220

Anti-Human CD45RA FITC
MBS696724-25Tests 25Tests
EUR 155

Anti-Human CD45RA FITC
MBS696724-5x100Tests 5x100Tests
EUR 945

FITC anti-human CD45RA
MBS9459532-25Tests 25Tests
EUR 165

FITC anti-human CD45RA
MBS9459532-50Tests 50Tests
EUR 200

FITC anti-human CD45RA
MBS9459532-5x50Tests 5x50Tests
EUR 755

Anti Dog Cd3:Fitc/Cd8:Rpe
GWB-Q01580 100 TESTS Ask for price

ANTI DOG CD3:FITC/CD8:RPE
MBS238973-100Tests 100Tests
EUR 595

ANTI DOG CD3:FITC/CD8:RPE
MBS238973-5x100Tests 5x100Tests
EUR 2510

MOUSE ANTI HUMAN CD45RA:FITC/CD8:RPE
MBS239276-50Tests 50Tests
EUR 520

MOUSE ANTI HUMAN CD45RA:FITC/CD8:RPE
MBS239276-5x50Tests 5x50Tests
EUR 2155

COMBI IC Reagent: Mouse anti Myeloperoxidase-C2 (FITC) and Mouse anti CD22 (PE)
MBS570348-1mL 1mL
EUR 615

COMBI IC Reagent: Mouse anti Myeloperoxidase-C2 (FITC) and Mouse anti CD22 (PE)
MBS570348-5x1mL 5x1mL
EUR 2620

CryoProtX Mix Reagents 1.5 mL
M-MDSR-61 1.5 ml ml
EUR 37
Description: CryoProtX Mix Reagents 1.5 mL

Rat CD3 (FITC) CD8 (RPE) Antibody
GWB-Q01606 100 TESTS Ask for price

Dog CD4 (FITC) CD8 (RPE) Antibody
GWB-Q01608 100 TESTS Ask for price

Mouse CD4 (FITC) CD8 (RPE) Antibody
GWB-Q01611 100 TESTS Ask for price

Mouse CD8 (FITC) CD3 (RPE) Antibody
GWB-Q01612 100 TESTS Ask for price

Set of 10 Biolipidure Reagents
Biolipidure-set 10mLx10
EUR 1820.4
Description: Set of 10 Biolipidure Reagents, whose applications include Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement

ANTI HUMAN CD4:FITC/CD8:RPE/CD3:RPE-CY5
MBS239222-50Tests 50Tests
EUR 520

ANTI HUMAN CD4:FITC/CD8:RPE/CD3:RPE-CY5
MBS239222-5x50Tests 5x50Tests
EUR 2155

REAGENTS HIGH RANGE AMMONIA - PK25
HI73325 PK25
EUR 55.35

High Range Ammonia Reagents - EACH
HI9373301 EACH
EUR 130.95

CD3 (FITC) / CD8 (RPE) Combination Antibody
abx410002-50tests 50 tests
EUR 760.8

CD8 (FITC) / CD4 (RPE) Combination Antibody
abx410006-50tests 50 tests
EUR 760.8

CD4 (FITC) / CD8 (RPE) Combination Antibody
abx410011-100tests 100 tests
EUR 744

CD4 (FITC) / CD8 (RPE) Combination Antibody
abx410012-100tests 100 tests
EUR 760.8

CD3 (FITC) / CD8 (RPE) Combination Antibody
abx410015-100tests 100 tests
EUR 744

CD3 (FITC) / CD8 (RPE) Combination Antibody
abx410018-100tests 100 tests
EUR 895.2

CD4 (FITC) / CD8 (RPE) Combination Antibody
abx410019-100tests 100 tests
EUR 895.2

CD45RA / CD45RO / CD3 / CD4 Antibody (FITC, PE, PerCP, APC)
abx200640-50tests 50 tests
EUR 828

CryoProtX Mix Eco Reagents 1.5 mL
M-MDSR-61-ECO 1.5 ml ml
EUR 37
Description: CryoProtX Mix Eco Reagents 1.5 mL

CD45RA / CD62L / CD3 / CD4 Antibody (FITC, PE, PerCP, APC)
abx200642-50tests 50 tests
EUR 828

CD4 (FITC) / CD8 (RPE) / CD3 (RPE-Cy5) Combination Antibody
abx410020-50tests 50 tests
EUR 744

FITC Anti-Human CD45RA (HI100)
MBS4165378-100Tests 100Tests
EUR 275

FITC Anti-Human CD45RA (HI100)
MBS4165378-5x100Tests 5x100Tests
EUR 980

PeliKine tool set, additional reagents
M1980 1 unit
EUR 169.8

COMBI IC Reagent: Mouse anti Myeloperoxidase-C2 (FITC) and Mouse anti CD3 (PE)
MBS570355-1mL 1mL
EUR 615

COMBI IC Reagent: Mouse anti Myeloperoxidase-C2 (FITC) and Mouse anti CD3 (PE)
MBS570355-5x1mL 5x1mL
EUR 2620

RAT ANTI DOG CD4:FITC/CD8:RPE
MBS238974-100Tests 100Tests
EUR 595
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